Issue with GC-MS for fatty acid profiling in maize??

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Simon Lagies

Hi Salina,

Could you please be a bit more specific what your problems are?

Salika Ramazan

Hello Simon,

Actually i tried to get fatty acid profile of maize leaf tissues using GC-MS analysis, but i am unable to get results.

I have incorporated 'heptadecanoic acid' as an internal standard in the extract during preperation, but thats also not visible in the GC-MS chromatogram, only noise appears.

Thanks

Simon Lagies Simon Lagies

Hi Salika,

There is indeed nothing to see...

1. Question: is your system working properly? Do other methods work, a quality Control Sample e.g alkane? Watch the Injection.

If everything is fine --> start esterification with your C17:0 alone. Signal? Then add the C17:0 always One step earlier in the protocol and tell us where you lose It.

@ Simon

Thank you for your suggestion.

Will see

Regards

Bruce Neagle

Be sure you use the FAME method of the AOCS (Association of the Oil Chemists Society). That is the same column, gases...

Otherwise you will have to accurately dry and grind a portion. Perform a Soxhlet extraction of the oil and proceed from there.

Dhia Falih Al-fekaiki

bio-protocol3014.pdf1.16 MB

Bruce Neagle Dhia Falih Al-fekaiki

Thank you for ur suggestions.

Warm Regards