Take 1g of rice rhizopheric soil and add 10 ml of sterilized water and mix it well. Then, prepare a serial dilution series.

Isolation of bacteria and fungi could be achieved using standard serial dilutions and spread plate methods.

Thank you Ganeshamoorthy Hariharan.

Take 10 g of rice rhizospheric soil into a 300 ml of conical flask and add 90 ml of sterile distilled water to it. Shake the flask for 30 min on a rotary shaker. Add 1 ml of suspension to 10 ml vial and shake for 2 min. Spread an aliquot (100 µl) of this suspension on Nutrient agar (NA) plated petridish. Incubate the plates at 28 ± 2°C and observe the growth of the fungus periodically. Streak bacterial colonies to other NA plates and incubate at 28 ±2°C for 3 days. Pick up the well-isolated single colony and re-streak to fresh NA plates to obtain single isolation and transfer to NA slants to make pure culture.