Isolation of RNA from Neutrophils?

10 May 2022 0 4K Report

Can anyone please help me out how to isolate RNA from cultured neutrophils isolated from human peripheral blood. I am using chloroform/phenol method. I got 10-30ng/ul ofRNA from 3-4 million cells. But on gel I didnot observe any band.

Thanks.

Badges
Science topic

More Hiral Thacker's questions See All

Is DNase treatment is necessary to RNA samples isolated from RNeasy mini kit from qiagen?

Is DNase treamtment is required to the RNA samples isolated from RNeasy mini kit from qiagen before doing one step real time pcr? Thanks

10 May 2022 4,649 1 View

Protocol for RNA isolation from culture neutrophils?

I am trying to isolate RNA from freshly isolated neutrophils from adult blood. Further I maintain culture for 3 hours hours according to my need. I use trizol choloroform method. I got very poor...

30 January 2022 2,803 3 View

How to design primer for qpcr spanning exon exon boundaries?

I selected the option "primer spanning exon exon boundaries in primer BLAST. Also put exon position in overlap position option in Primer 3 and IDT but unable to design a good primer. Every i got...

23 January 2022 1,601 10 View

How to calibrate Qual2kw software?

I am using Qual2kw software in my research work for water quality modelling.I am not able to understand how to calibrate model either use VBA or fortran programme with giving weight and...

24 May 2020 8,284 4 View

Anyone have an easy MS excel template to share for Reaction Engineering Approach modelling of dairy powders?

26 July 2018 3,159 1 View

When x% ZnO added in LSMCO sample, magnetization was decreased, What is the reason behind that mechanism?

1st, I have prepared LSMCO sample and then after I have added x% ZnO in LSMCO sample and make it composite. My confusion is that in magnetic properties, Magnetization of composite sample...

29 May 2018 804 2 View

How is resulting initial contact stiffness calculated in Ansys workbench?

What are the various factors on which the resulting initial contact stiffness depends in Ansys workbench.

04 February 2018 7,922 2 View

What is the variation in resulting initial contact stiffness when we replace a thin solid model with surface model in Ansys Workbench?

how contact stiffness varies when we are using shell elements (shell181) in place of solid elements (solid 186) for thin models in ANSYS Workbench?

21 January 2018 3,974 0 View

Design of Sequential Moving Bed Biofilm Reactor.

Is it possible to combine SBR and MBBR in one reactor as an SMBBR for the treatment of the domestic sewage?

08 August 2017 7,671 4 View

How to controll shape of ferrites ?

How to synthesize ferrites of various shape like spinal,elliptical, wire, flakes etc. in nano or micron particle size. which factors determine the shape of ferrites In co-precipitation or other...

13 July 2017 6,045 3 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Where to find a gene list for CRISPRa/i library screening of regulatory factors that affect pathogenic Th17 differentiation in PBMC?

I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...

31 July 2024 2,150 0 View

Is it common for heterotrophic bacteria to grow on Pro99 and SNAX media?

I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...

30 July 2024 1,040 1 View

How to remove/ replace trifluoroacetate countertop after deprotection?

After deprotection of the tert-butyl ester to generate the carboxylic acid in chloroform with TFA, my compound‘s pyridine moiety forms a TFacetate ammonium salt (small molecule drug, not a...

29 July 2024 9,753 3 View

Question about MHB (Mycorrhiza Helper Bacteria) isolation technique??

What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.

26 July 2024 7,387 0 View

Question about Single Strain Arbuscular Mycorrhiza Fungi??

Now i am working on AM fungi single strain. What are the most effective isolation techniques for obtaining pure cultures of arbuscular mycorrhizal (AM) fungi?? Is there are best approach...

26 July 2024 6,520 1 View