Hiral Thacker

3 Questions 3 Answers 0 Followers

Questions related from Hiral Thacker

Is DNase treatment is necessary to RNA samples isolated from RNeasy mini kit from qiagen?

Is DNase treamtment is required to the RNA samples isolated from RNeasy mini kit from qiagen before doing one step real time pcr? Thanks

11 May 2022 4,652 1 View

Protocol for RNA isolation from culture neutrophils?

I am trying to isolate RNA from freshly isolated neutrophils from adult blood. Further I maintain culture for 3 hours hours according to my need. I use trizol choloroform method. I got very poor...

31 January 2022 2,806 3 View

How to design primer for qpcr spanning exon exon boundaries?

I selected the option "primer spanning exon exon boundaries in primer BLAST. Also put exon position in overlap position option in Primer 3 and IDT but unable to design a good primer. Every i got...

24 January 2022 1,603 10 View