Hiral Thacker

5 Questions 4 Answers 0 Followers

Questions related from Hiral Thacker

Hello, I am trying to Isolate exosomes from human body fluids . Iam using total exosome isolation kit (invitrogen),I have a couple of questions?

1. Firstly, I am not getting any pellet even after following manufacturer's instructions. 2. I lysed it with 8M urea and 2% SDS and got 30ug/ul protein after estimation with bradford assay. Is...

04 December 2024 3,622 4 View

Is DNase treatment is necessary to RNA samples isolated from RNeasy mini kit from qiagen?

Is DNase treamtment is required to the RNA samples isolated from RNeasy mini kit from qiagen before doing one step real time pcr? Thanks

11 May 2022 4,673 1 View

Isolation of RNA from Neutrophils?

Can anyone please help me out how to isolate RNA from cultured neutrophils isolated from human peripheral blood. I am using chloroform/phenol method. I got 10-30ng/ul ofRNA from 3-4 million cells....

10 May 2022 4,449 0 View

Protocol for RNA isolation from culture neutrophils?

I am trying to isolate RNA from freshly isolated neutrophils from adult blood. Further I maintain culture for 3 hours hours according to my need. I use trizol choloroform method. I got very poor...

31 January 2022 2,831 3 View

How to design primer for qpcr spanning exon exon boundaries?

I selected the option "primer spanning exon exon boundaries in primer BLAST. Also put exon position in overlap position option in Primer 3 and IDT but unable to design a good primer. Every i got...

24 January 2022 1,628 10 View