Isolation and purification of microalgae from river ?

More Lawrence Vitug's questions See All

Is Spirulina species growing on BG-11 medium ?

I have tried it but doesnt work, im trying zarrouk medium and it seems working well. Im trying the medium by Andersen (Spirulina Medium (Aiba and Ogawa) Hope it works, I got the Spirulina from UTEX.

03 April 2017 4,028 7 View

Can anyone suggest a procedure for the SEM of Botryococus?

I have already isolated Botryococcus species and optimized the culture conditions, I need to identify it base on SEM and LM microscopy and later on phylogenetic analysis, Your help will be greatly...

06 July 2016 8,245 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

What is the most suitable medium of ascomycetes fungi ??

26 February 2021 4,413 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Eldrin De Los Reyes Arguelles

You can use nystatin against fungi..for protozoans you can do serial dilution or do streak plating...

Lawrence Vitug

Thanks for the info Alfonso and Eldrin, What about the concentrations and volumes (mg/ml or ug/ml) how many mg or ug per ml or liter.

Mohammed Mahmoud Essam Ghobara

use 50 mg Nystatin per liter and increase the dose if the infection is higher. for protozoans use streak plating! that's nothing comparable with fungi and bacteria