Dear Assraa Hassan Jassim,

I assume that you are informed about the duration of the procedure of determination of protein carbonylation. Concerning the lipid peroxidation, I would like to cite a known opinion about it:

"In contrast to lipid peroxidation the products of which typically appear after a lag time, protein damage by reactive oxygen species takes place directly and immediately. The most common drawback of protein degradation assays is the complexity of the applied methods such as radioactive or fluorescent labeling, gel electrophoresis, Western blots, or immunoprecipitation".

(Pacifici, R.E., Davies, K.J.A.: Protein Degradation as an Index of Oxidative Stress. In: METHODS IN ENZYMOLOGY, 1990, Vol. 186, Part B, Eds. Packer, L. and Glazer, A.N., Academic Press, New York, p.p. 485-502). We developed a method, based on two different measuring principles, which is free of these disadvantages (US patent 7,125,723 B2 and two publications are attached). The preparation of a blood sample for determination of the oxidativen modification of plasma proteins lasts 10 minutes, the measurement 2...3 minutes. The detection of oxidative modification of nucleotides and nucleic acids is possible too (4th attachment).

With best regards,

I. Popov

Dear Dr. Popov,

Thank you so much for your insight. I highly appreciate the attachments and I am reviewing them. My main focus is to detect oxidative stress in retina and optic nerve of a mouse model of glaucoma.

Sincerely,

Assraa

definitely it differs based on the used method of analysis and i think it is hard to find one buffer for all oxidative stress biomarkers. dr popov attachments answers such misunderstandings.