Is THPS toxic towards methanogenic bacteria? In case it is, is there a "selective (more or less!) biocide against sulphate reducing bacteria?

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Issues with my microglia primary culture?

Hi everyone, I'm isolating microglia from P1-2 mice and seeding microglia cells from 3 pups brains/T-25 flask. After seeding, I let this astrocytes-microglia mix culture grow for approx 2-3 weeks,...

06 October 2020 3,916 3 View

Do you have any suggestion for a easy and cheap experiment to quantify Cytokines?

I need to test the stimulation production of TNF alpha in the RAW cell line by LPS. I am looking for an easy and cheap experiment to quantify the serial dilution stimulation in this macrophage...

27 September 2020 5,366 6 View

Good antibodies for D1 and D2 striatal medium spiny neurons?

Hello! I am looking for good antibodies that help in clearly distinguish between D1- and D2-type medium spiny neurons (MSNs) in the striatum, by immunohistochemistry and immunofluorescent...

31 May 2020 5,009 3 View

Good antibodies for rat M1 and M2 microglia?

Hello! Do you know some good antibodies, suitable for IFL, for differently staining the M2 and M2 microglia phenotypes in rat tissue? I am searching for antibodies specific for RAT because all the...

27 October 2019 7,260 3 View

Anyone can help me about grayanotoxin analytical standards for LC-MS/MS?

I need to buy analytical standards for grayanotoxin I, II and III. I only find the type I. Roberta Merlanti

15 May 2019 2,628 4 View

Could someone help me to choose the right concentration and time of neutralizing IL1beta antibody after LPS treatment to block its signalling?

Thanks!

06 October 2016 4,648 3 View

Any alternatives to 3MA solubilization?

I have to use 3-MA in my cell culture at high concentration. II know that 3-MA is not very soluble in water, so the solution would be solubilize 3-MA in DMSO, but I know that DMSO generally...

08 June 2014 1,658 3 View

What types of transfection reagents do people use to transfect neurons, IPS derived neurons, astrocytes and neural stem cells?

Do more people use cDNA, siRNA, mRNA or miRNA? Would love to hear what people are using?

29 January 2014 1,147 7 View

What types of transfection reagents are you using to transfect neurons, astrocytes, IPS neurons?

cDNA, mRNA, siRNA, miRNA

27 January 2014 4,591 3 View

Has anyone used Dasatinib to revert grade IV ICANS after KTE X19 (or AXI cel) use?

Our patient is refractory after 3 lines of ICANS post KTE X19 treatment for MCL (dexamethasone, methylprednisolone, anakinra). Has anyone used Dasatinib for neurotoxicity in post CAR T cell use?...

01 January 1970 2,854 1 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

How to dilute Toxin-DMSO solution?

I have a concentrated solution of a toxin(50 micromolar) in DMSO 100%. I need to dilute it inorder to dilute both the toxin and DMSO wich can be toxic to cells. Which would you guys recommend to...

19 February 2021 7,447 2 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...

09 February 2021 4,994 1 View

Review Papers regarding transcriptional regulations & promoter types/classes in Gram positive bacteria?

Dear fellow researchers, Could you please suggest me excellent review papers discussing transcriptional regulations and/or promoter classes in Gram positive bacteria? Thank you so much for your help!

08 February 2021 6,909 3 View

Has anybody administered MK-2206 (an orally active, allosteric inhibitor of AKT) intravenously to the mice?

We aim to use MK-2206 to inhibit AKT in mice. However, a large body of studies suggests that it should be administered orally. I was wondering if somebody could tell me that is it toxic for the...

07 February 2021 6,340 1 View