Hi everybody
I'm trying to record some sodium current in cortical neurons. However during the activation protocol I have some weird outward current, even with TEA+ 4AP solution
Does my current look as it should
Hello: I am looking for articles that address e-commerce businesses and COVID-19 survivors, as well as Entrepreneurial strategies that reflect e-commerce businesses. Blessings Evelyn Dempsey
05 August 2024 5,332 3 View
A typical reforming section in a large-scale Haber-Bosch process (Figure 1) comprises two reactors. A mixture of methane (CH4) and steam (H2O) is fed into a primary reformer to produce syngas via...
01 August 2024 7,051 5 View
How can you regulate the number of random inserted mutations in error prone PCR?
14 May 2024 673 2 View
I just joined to ResearchGate but I realized that several of my publications are linked to other "Marcos Andrade (Universidade Presbiteriana Mackenzie)". Please help me claim my work
14 May 2024 1,053 1 View
Electrochemistry is a pathway to convert CO2 into valuable products such as CO. However, a potential side product in the cathode is H2. I wonder if it is possible to tune the composition of the...
12 May 2024 9,061 3 View
Hello! I am currently running a western blot using human AD tissue samples. I prepared these samples over a year and a half ago. I am having trouble standardizing these proteins with Beta Actin. I...
02 May 2024 9,588 3 View
I am currently running a plasmid isolation. I am using a bacterial stab to grow the plasmids. We have used this stab a few times now. Unfortunately, we have not had the chance to make glycerol...
08 April 2024 2,035 4 View
Pulsed electric field is considered a "non-thermal" method for food processing that involves the application of short high-voltage pulses to create pores in cell membranes. However, since I am not...
13 March 2024 2,624 6 View
I read that potassium clavulanate is water soluble. I tried to dissolve it to a very low concentration but the liquid remains cloudy and a white powder is visible at the bottom of the vial
06 March 2024 8,900 0 View
I’m concerned about the dots on my MEF cells and I’m worried that it could be contamination.
31 January 2024 5,963 0 View
Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...
23 July 2024 6,314 3 View
I need to make a stock solution for radioisotopic incubations of freshwater sediments to study dark primary productivity but I have some confusion as to how I calculate the concentration of 14C...
15 July 2024 5,898 0 View
During the release test of Amphotericin B using 1% Tween 80, i observed three peaks even in the standard. The first peak consistently yielded results around 500%, while the other peaks showed...
08 July 2024 1,330 0 View
I did some column tests in my lab where I needed to detect the DOC fractions using LC-OCD. For the abiotic columns I used sodium azide to restrict the biofilm formation. I did LC-OCD to detect the...
07 July 2024 468 0 View
Hello All, I've been facing difficulty in executing geometry optimization of a sodium polyanionic phosphate (Na4Fe3P4O15) crystal in CASTEP (please find the input and output files attached). When...
07 July 2024 8,626 2 View
Can this pH of 8 be due to the presence of other substances? Or do I have an error somewhere in the analysis? Thank you!
26 June 2024 9,093 4 View
Flavors of all tea leaves are determined by their respective chemical compositions. Are there any quantitative research on this?
25 June 2024 2,553 2 View
I have been trying to grow Nicotiana benthamiana via tissue culture. I used ethanol and sodium hypochlorite with different concentrations and time duration for seeds sterilization and lastly...
18 June 2024 1,756 3 View
I want to replicate the third step, but there seems to be no reaction in an alkaline environment. However, sodium hydroxide is used in the literature. Should I switch to an acidic environment
05 June 2024 9,358 1 View
blue tea from butterfly pea plant
03 June 2024 2,558 1 View