If some one is designing a vehicle for any kind of drug delivery, is this necessary that it should show anti microbial activity by itself? If it is so, then how to differentiate between the activity of drug and the vehicle?
It is always recommended to use vehicle which is inert ( or has no activity). However, if you end up using an anti microbial property exhibiting vehicle, then in your experiment, along with drug attached vehicle also use vehicle by itself ( without drug or bare vehicle). In this way all the false positives can be nullified.
NO. The purpose of a delivery vehicle is to protect the active drug from degradation (by the contents of gut/blood) and to facilitate delivery of the drug to the target tissue in a pre-determined fashion. Of course the delivery vehicle could be functionalised with a variety of ligands (eg antibody to a surface antigen) for targeted drug delivery or to reduce clearance by RE system (basic ideas of nanopharmaceuticals).
Hope that helps you.
Siva
Why would someone think that a carrier needs to show a certain type of biological activity? Are you concerned about its sterility?
@ Riaz A. Khan- Because I am working on edible vehicles from natural resources. And they must not get infected with microbes.
The forum's convention is to write without personification. Need to be spelling out the complete query to let the paraphernalia play out fully in its answer by viewers! The problem's question and answer belong to the sterile conditions of preparation rather than the bioactivity of the carrier.
It's not necessary but I guess it's important that you evaluate it alone and with the drug because maybe the delivery system is affecting the antibiotic efficiency of the free drug. I mean, you have to test free vehicle, free drug and different proportions of the vehicle:drug.
In my opinion, you should choose the vehicle having no antibacterial activity. In this way, you can better describe the efficiency of the drug loaded in NPs. But if you have the vehicle having antibacterial property then do your experiment with drug-loaded NPs (lets say zone of inhibition is 35mm) and only NPs (15 mm) then subtract 35 mm from 15 mm indicating the antibacterial efficiency of the drug.
I guess sometimes you are searching for your material to enhance the antiobiotic effect so the most straightforward thing to do is to evaluate empty material, free antiobiotic and the combinations. That was my undergraduate thesis.
Thesis Determinación de la eficacia antibiótica de los complejos de...
Dear Varun Saxena, an ideal carrier should be inert. However, current research is looking for non-antibiotic antibacterial agents due to problem of MDR. It is good if your nanoparticles are antimicrobial. You should study them without drug or to synergise antibiotic activity (and compare with a reference antibiotic). In either case, you will be required to measure antimicrobial activity of vehicle alone. However, remember that if a nanoparticles can enhance therapeutic activity of a compound, same should be expected about its side effects. This is especially true for nanoparticles/nano-vehicles that act on cell membrane or DNA of bacteria.
Regards
Mubashar
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