The enclosed figure is an fEPSP trace I recorded from rat motor cortex, with the recording electrode placed at layer II/III and the stimulating electrode at layer V (stimulating the vertical afferent fiber coming from white matter).
I always thought that with a negative-going fEPSP curve, the inverted trough preceding the negative-going fEPSP reflects a presynaptic fiber volley. Recently I read an article (Dasari and Yuan, Toxicol Appl Pharmacol, 2009, 240: 412-422; done in visual cortex with identical stimulation and recording arrangement) saying that this is a population spike caused by antidromic stimulation of the axon. I believe that my stimulation method should be orthodromic. How did I achieve antidromic stimulation? So I am confused. Which one is it ? a fiber volley or population spike? Hope someone can give me an answer.
I would like to know why and how to tell the differences. Thanks in advance!
This is the figure copied from Dasari and Yuan (2009), stating that the first trough preceding the fEPSP is antidromic poppulation spike (anti-PS)
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