Is this a comet ? - FAQS.TIPS

27 February 2025 1 9K Report

Good evening,

This is my first time experimenting with the comet assay. Specifically, we are running PBMC samples and using silver stain for visualization. Could this image observed under the light microscope (40x objective) be a comet that can be evaluated?

Thank you very much in advance!

Malcolm Nobre

Good evening Panagiota Resta

No. The article attached below will help. Have a look at Fig 1.

https://ri.conicet.gov.ar/bitstream/handle/11336/128665/CONICET_Digital_Nro.764aa6e7-483f-46ae-8baf-97919693ba5e_A.pdf?sequence=2&isAllowed=y#:~:text=THE%20SiNGLEHCELL%20GEL%20ASSAy%20(also,(Ostling%20and%20Johanson%201984).

Best,

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