I've separated total lipids from the milk and intended to make liposomes out of it, can anyone guide me on which of the method is the most appropriate for total milk lipid liposomes preparation.
No, the microfluidization and heating method techniques have high entrapment efficiencies. Few articles are attached for you to consult:
Article Preparation of Liposomes from Milk Fat Globule Membrane Phos...
DOI: 10.1051/lait:200701
DOI: 10.3168/jds.S0022-0302(06)72105-1
Check https://www.sciencedirect.com/science/article/pii/S0022030206721051
Also check https://www.intechopen.com/books/molecular-pharmacology/liposome-a-comprehensive-approach-for-researchers
Riaz A. Khan
There are using MFGM as a source for liposomal preparation, my source is whole milk-derived fat.
Greetings,
Better to avoid techniques which are harmful to users and environment. New techniques have eliminated need to use "ORGANIC SOLVENTS" (e.g. chloroform, methanol) or "HIGH PRESSURE PROCEDURES" such as homogenization or microfluidization.
Have a look at methods described in detail in the attached literature:
Mozafari, M. R. (2010). Nanoliposomes: preparation and analysis. In Liposomes (pp. 29-50). Humana press.
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