Hi, I'm using Di-4-ANEPPS for voltage sensitive fluorescence imaging.
and based on my study,
I began to doubt that some spectral shifts were occur by change of solvents.
But I cannot find any quantitative information about shifting of spectrum based on solvents.
Is there any kind of articles that has information about emission spectrum chage(shift) of VSD varying from kinds of solvent (EtOH vs DMSO)?
Gopal Singh
Apart from polarity difference, ethanol and DMSO are protic and aprotic solvents respectively and have huge difference in their dielectric constant and pi star value. This difference in nature can significantly alter the fluorescence behaviour of any molecule. You can get numerous papers on solvatochromism in literature.
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