Is there is any protocol for preparation of chemical competent cell of staphylococcus aureus ?

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Kazi Tawsif Ahmed

chemical transformation in gram positive is not possible. you have to go for electroporation only.

Anwar A. Abdulla

For bacterial species that are not known to be naturally competent, such as Staphylococcus aureus and Staphylococcus epidermidis, electroporation is an efficient method for introducing genetic material into the cell.

Article Method for Preparation and Electroporation of S. aureus and ...