Sudipto Kumar Paul

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Questions related from Sudipto Kumar Paul

Why I'm getting multiple bands after Ni-NTA based protein purification?

I'm working with some short protein with size around 20 kDa. I'm expressing the protein in E. coli BL21(DE3) system at 25 degree, 150 rpm in YT media. While I'm checking the proteins in cell...

18 March 2020 2,462 3 View

Is there is any protocol for preparation of chemical competent cell of staphylococcus aureus ?

I have been trying to transform my isolated plasmid from DH5a to Staphylococcus aureus. I have tried electro competent transformation, but unable to do that. Is there any method for chemical...

18 March 2020 9,823 2 View