i have developed a bioinformatic tool (Link: geltowgs.uofk.edu). that compares PFGE gel image analysis results to mathematical models of band sizes derived from WGS (FASTA files). I have suggested a new algorithm to count DNA fragments that co-migrates across each lane. We are about to publish ower work (Research gate DOI: 10.13140/RG.2.2.32752.76806) . The attached file illustrate our method.
thank you
@Moussaab Belarbi
thank you very much for your answer. that tool predicts a PFGE pattern from WGS but my tool compares a real PFGE image analysis results to the models derived from WGS sequences. We had to figure out a method to calculate how many different fragment accumulate in each band and we successfully found that method(described in the attached file).
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