Is there extrasynaptic Synaptophysin?

21 September 2021 1 8K Report

Dear peers,

I wondered whether Synaptophysin is exclusively located in the presynaptic terminal, or it could also be observed elsewhere in the neuron?

Thanks!

Amjad Hasan Bazzari

Dear Vladimir Khayenko

Early studies showed that synaptophysin (SY) is restricted to vesicles and Golgi tubular membranes:

https://journals.sagepub.com/doi/abs/10.1177/39.11.1918927

Evidence using the confocal microscope showed that most SY is identified as axon terminal vesicles

https://www.sciencedirect.com/science/article/abs/pii/S0165027099001636

Since it is a vesicle protein, you would expect to find it wherever there are vesicles. You may look through studies that stain SY in neurons like the following paper:

https://content.iospress.com/articles/journal-of-alzheimers-disease/jad160297

As for extrasynaptic locations, the following paper is very interesting to read as it involves vesicular tracking through monitoring SY.

https://www.sciencedirect.com/science/article/pii/S258900421930197X

Badges
Science topic

Similar topics
Eutheria
Rodentia
Muridae
Murinae
Mice

More Vladimir Khayenko's questions See All

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Cox regression baseline hazard?

Dear colleagues, I would like to make a mathematical equation of Cox regression model, however I am stuck with baseline hazard. I clearly understand interpretation of coefficients, but I can't...

24 February 2021 6,207 3 View

How to calculate the electric field in the rotating frame (the question in the general relativity)?

In the classical electrodynamics, there is one problem - to find the EM fields created by the charge moving with the constant velocity in the circular orbit or in the helical path (free electrons...

12 January 2021 3,689 1 View

Do you know anything about Elena Bokus, the early 20th Century Architect / Architectural Designer?

The available research of early 20th century history of architecture in North Macedonia, suggests that probably the first female architect / architectural designer was Elena Bokus (born in 1893...

23 December 2020 2,039 2 View

Can you recommend a short and free Deep Learning course that is sufficient to enable implementation on Java?

It would be best if the material is available on the web, a pdf version is also possible. If you still have knowledge of a good video course, please share.

29 November 2020 4,769 2 View

Why the Helmholtz decomposition theorem is not applicable to the electrodynamics?

My question is caused by one curious fact of the electrodynamics. It is commonly accepted opinion that the electromagnetic fields can at least formally be decomposed onto the rotational and...

10 September 2020 8,668 3 View

COS7 cell transfection - do you have a good protocol?

Dear all, lately we ran into trouble with COS7 cell transfection, we get low transfection rate with the protocol we use. Do you have a good transfection protocol? Preferably Calcium transfection...

23 May 2020 842 2 View

Can an electron and a hole have one common coordinate y_0 of the center of the quantum orbit in a two-dimensional semiconductor in a magnetic field?

Can an electron and a hole have one common coordinate y_0 of the center of the quantum orbit in a two-dimensional semiconductor in a magnetic field? (if they have the same or opposite directions...

16 January 2020 5,009 8 View

What is the density of excitons on a two-dimensional semiconductor in an external magnetic (and without magnetic) field?

What is the average density of excitons on a two-dimensional semiconductor in an external magnetic (and without magnetic) field? What is the dependence of this density on the magnetic field B?

12 January 2020 173 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How can I stain the somatodendritic part of inhibitory neurons in a hippocampal culture with high specificity?

I would like to distinguish the somatodendritic part of the inhibitory neurons in a hippocampal culture (where we expect to have 5-10% of GABAergic neurons). What are the best antibodies and...

23 February 2021 6,756 1 View

The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

21 February 2021 8,945 3 View

What are alternatives for B27+ Supplement in neuronal cell culture?

Hello everybody In our iPSC-derived neuronal culture we use Maturationmedium, supplemented with B27+. As Thermo Fisher run out of stock and can deliver earliest in May, I wanted to ask whether...

16 February 2021 3,348 1 View

How to reduce debris with neuron isolation?

Hi there, I'm isolating neurons from PND1 guinea pigs and have noticed a bit of debris in the early days (this is day 4 plated) and a lot of variability between plates. Current protocol is...

14 February 2021 3,692 4 View

Does anyone have a stack of images of action potentials or abnormal brain scans?

I need a stack of black and white .jpeg/.tiff/.jpg/.png images across time of either action potentials, neurons firing, or brain scans (comparing disease and normal brain, disease progression,...

09 February 2021 2,648 3 View

Do head direction cells necessarily encode in an allocentric reference frame?

According to the vertebrate literature, I got the impression that head-direction cells are always anchored to an allocentric reference frame. This can easily be tested with freely moving animals....

27 January 2021 3,821 3 View

Anti-Src and anti-pY418 Src antibodies for IF?

Hi everyone, Does anyone have a suggestion for anti-Src and anti-pY418 Src antibodies that work well in immunofluorescence on cultured neurons? Thanks a lot!

26 January 2021 866 1 View

Does the pre-synaptic neuron location in a cortical map influences the spatial position of its synapses in the dendrite of the downstream neuron?

Are functional maps in the cortex used by the brain to carry out computations or are they just a byproduct of wiring minimization? A key element to answer this question is to know if, when...

26 January 2021 1,118 8 View

Any advice on selecting human or animal studies to conduct a systematic review?

Human studies are more relevant to the project, however there are more mouse studies because it is mostly carried on neuron progenitor cells to identify the effect of a virus in the development of...

11 January 2021 5,628 3 View