Is there evidence in any model system of RNA (micro or larger) uptake either by neighbouring or distant cells?

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

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02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

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Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

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Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

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Where to find data on trees in tropical agroforestry?

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28 February 2021 7,356 8 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

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28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Tabari Baker

Absolutely! There is an abundance of evidence describing the shuttling of microRNA and messenger RNA through microvesicles and/or exosomes. I am most familiar with microRNAs, so my answer will focus on those. The most well characterized model system of exosome-mediated microRNA transfer in the immune synapse between dendritic cells and T-cells (see attached paper by Nolte-‘t Hoen et. al.). There is also significant evidence of both exosomal transfer of microRNAs in several cancer-related mechanisms (see attached papers by Tadokoro et. al. and Hood et. al.). Finally, some recent evidence has shown that microRNAs can be selectively packaged into exosomes for secretion through the recognition of a sumoylated RNA-binding protein to a specific microRNA sequence (see attached paper by Villarroya-Beltri et. al.).

Sorry for the long answer, but I hope it helps. The additional attachments will be added as separate comments.

Barak Rotblat

A nice one as well.

Cell contact-dependent acquisition of cellular and viral nonautonomously encoded small RNAs.

Rechavi O, Erlich Y, Amram H, Flomenblit L, Karginov FV, Goldstein I, Hannon GJ, Kloog Y.

Genes Dev. 2009 Aug 15;23(16):1971-9. doi: 10.1101/gad.1789609.

María Mittelbrunn

Transfer of microRNAs during immune syanspes.

I hope this helps you.

Article Unidirectional transfer of microRNA-loaded exosomes from T c...

Ashis Das

Thanks for all the information. I am sorry missed the early replies. My focus is on malaria and this does further the possibilities of host -parasite modulation in either direction. Could extend to many other similar infections as well. Would certainly have implications for preventives being investigated.