In human 1000 genome project decoy sequence is used for absorbing reads which do not truly belong/map to the reference, but are often mapped. Are there similar decoy sequences designed and used in rice genome (reference based) assembly ?
We were fortunate enough to recieve a plasmid from abroad. It was spotted on a filter paper and sent to us. We are eluting the plasmid after almost 1.5 months. We added 30 ul of TE buffer to the...
14 November 2022 4,555 3 View
Hi, i'm trying to prepare HaCaT cell lysate for biochemical assay studies. However, my protein concentration are too low (e.g 0.3mg/ml). My protocol is as follows: 1. Cultured HaCat in 6-wells...
30 November 2021 6,038 1 View
Multidimensional scaling (MDS) is used by multivariate data to create distances and analyze, similar to analyzing the data using categorical principal components analysis of normalized data. What...
31 May 2020 3,175 2 View
Methods that are available for extraction of Zinc from Flue gas cleaning residues, especially of Iron and Steel Sector. What are the success ratio and purity of zinc that are extracted?
08 September 2019 4,138 1 View
Sono catalytic and be coupled with ozone as advanced oxidization, what kind of the mechanism will take place when we couple microbial treatment with sonocatalyst. Is the growth of microbes get...
17 December 2018 6,143 4 View
I stained the cells with mouse CD45.2-PE antibody to the single cell suspension prepared from mouse bone marrow. Even after adding twice the recommended concentration, I am still seeing the...
16 September 2018 9,162 1 View
I am beginner in quantum chemistry, pursuing my research on structural activities of secondary metabolites. While performing antioxidant mechanisms, I am using gas phase enthalpy values of proton...
09 August 2018 3,918 3 View
I prepared 10% CTAB in 0.7M NaCl and at room temperature it is still in liquid form but l learnt from colleagues that it forms a gel at room temperature. Will it impact the yield in DNA...
23 July 2018 1,786 1 View
I am trying to use a ageing equation from one of the models available in literature. I find that if I use the same constants provided by the authors, the total number of cycles is around 200 for...
13 November 2016 7,224 6 View
I have executed a optimization algorithm 'A' towards an objective.After 50 independent runs i have got 50 values.I have executed a optimization algorithm 'B' towards the same objective.After 50...
03 July 2016 6,904 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
What is the prepared reference material that can be used in the ICPE-9820 Shimadzu Japan instrument, which employs inductively coupled plasma optical emission spectrometry (ICP-OES) to measure...
06 August 2024 1,896 1 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...
03 August 2024 2,484 1 View
Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...
01 August 2024 1,514 1 View
I am a student in a master's program for Special Education and I am curious to see what Evidence-Based Practices that are used in teaching individuals with Autism Spectrum Disorder benefit from...
30 July 2024 5,611 1 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...
28 July 2024 8,953 1 View
Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...
23 July 2024 2,673 6 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View