Or can you only rely on the application recommendations. I'm trying to understand how much of the actual antibody I am purchasing. Some antibodies say 100µg, others say 100µl.
In the case of 100 µl, you need the respective concentration (usually it is given somewhere in the datasheet). 100 µg is 100 µg, at least in theory. These numbers are only estimates: the amount could be lower, due to impurities, or higher, if the company wants to be on the safe side. Very difficult is the question of the amount of active antibody. This fraction might decrease significantly, e.g. by freezing, lyophilization, storage and so on. In addition, the amount of antibody is not the only information you need. Some poor antibodies need much higher working concentrations as good antibodies, which can be used in a highly diluted form. Usually, quality is more important than amount.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4803150/
The above answers are good if you can find the data use it. Otherwise you can take a microlitre and nanodrop it. Use the protein function with IgG as your choice to calculate (estimate) amount. Gives one a pretty good idea. OR run a microlitre out on a gel and compare it to known Gamma globulin amounts or known IgG amounts as standards. Simply by Coomassie stain.
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