I have an urgent dissolution issue.
I have a stock solution of br that I bought from Sigma. I checked the papers regarding the solution buffer. Is there any recommendation?
I don't have any experiences with your molecule, but with other apolar/lipophilic proteins. Since your protein consists of 7 trans-membrane helices, it should have a lipophilic character, too. Therefore, you should be able to solve it in apolar organic solvents, .e.g. DMSO.
For transmembrane proteins, a detergent is usually used to make a solution. According to the Sigma web site, you can make an aqueous suspension by sonication.
I used ph 9.4 buffer (it was recommended in a paper) I couldn't get dispersed solution.
Also water did not work.
This is a problem. I've worked with the Sigma preparation and as I remember and Dr Lazarova reminds me - it is a crude membrane preparation. For our purposes, film preparation for experimental optical storage, we sonicated it in a cholate buffer. This gave an acceptable solution for our purpose,
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