I can buy even if it is not in good condition as I am ready to fix the minor adjustment. Please I am in dire need of it.
Hello everyone ! I was wondering how long one could freeze cell lysate (at -80°C) in TRIzol prior to DNA extraction ?
10 September 2023 4,390 3 View
I want to calculate Area per Lipid using the VMD membrane plugin, and I need specific carbon atoms for the calculation. How do I select carbon atoms for calculating Area per Lipid? How do I know...
12 February 2023 6,447 0 View
Has anyone ever observed a percent identity of the order of 97% between two operons of the intergenic region 16S-23S rRNA in a same cell ? Do you have some references ?
08 January 2023 7,882 3 View
I would like to get a script written in biopython that creates a list of genome accession numbers for the genus Pseudomonas. But I don't know enough about biopython to achieve this. The goal is to...
28 December 2022 4,424 5 View
Hi, I'm trying to induce apoptosis in hemocytes in suspension from bivalves. For detection, I use the CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit from ThermoFisher to mark apoptotic...
01 December 2021 7,139 2 View
Hello everyone! We bought the TOPO™ TA Cloning™ Kit for Sequencing, with One Shot™ Mach1™ T1 Phage-Resistant Chemically Competent E. coli from ThermoFisher. Unfortunately, the competent E. Coli...
26 August 2021 1,656 2 View
Hi I´m planning on estimating pore size induced by antimicrobial peptides in liposomes by using FITC dextrans. However, I can´t seem to find the estimated Stoke´s radii for FD10S, FD40S, FD70S,...
10 June 2021 7,373 0 View
Hi, I have been looking around for ways to insert various monomers of an AMP in membrane bilayers. To start-off, I have been using Charm-Gui, but it seems that I can only insert one peptide at a...
29 November 2020 8,822 2 View
I had hoped to run a workshop to understand how militaries and humanitarians should share clinical best practice (or indeed IF they should). It was intended to be a workshop over a day or two - a...
01 July 2020 1,030 2 View
Covid-19 is confirmed also an air-borne disease. This means staying indoors may not be safe. For example, a gust of wind carrying the viruses blowing into the house or anywhere in the environment...
06 June 2020 2,677 3 View
My colleague and I started research project about bioplastic made out of starch-curcumin composite. However, we are currently having trouble regarding the produced film. We have degassed the...
01 July 2024 9,419 0 View
Hello everyone, I am first year PhD student, and I should work with Qupath for part of my project ( for cell detection and quantifying), I watched many tutorial videos, I asked from AI, I sent an...
20 June 2024 8,886 0 View
Aquatic insects
11 June 2024 8,144 3 View
Hi everyone, I've created a p-chart to monitor a binary variable on a quarterly basis. But I noticed a p-chart requires at least 20 data points. So, I've shifted to monthly reporting. with this...
06 June 2024 4,849 0 View
after tafel extrapolation, is there need to do more adjustment?
01 June 2024 2,780 0 View
Hi, I am facing a temperature convergence issue in Silvaco Atlas. At the threshold voltage the temperature is not converging. I applied given models models consrh bgn FLDMOB HCTE MASETTI...
02 May 2024 3,059 0 View
I'd like to ask you to provide me with some models for assessing young people's adjustment to university, considering different dimensions (e.g. academic, mental health, wellbeing, interpersonal)....
23 April 2024 2,456 0 View
i need some information to build a thesis about minors community
22 April 2024 1,081 0 View
hi I am planning to use tissue carriers consisting of two rings (base and tension) that can trap tissue or membrane in between them so I can utilize both surfaces of membrane (image attached),...
15 April 2024 9,832 0 View
How does Earth's surface get heated without the presence of greenhouse gases in the atmosphere and greenhouese gases trap heat in the atmosphere, and what effect does this have on climate?
14 April 2024 6,504 2 View