What is causing the raw fluorescence to be negative?

Hi everyone, I would really appreciate it if someone can tell me when and why a raw fluorescence starts below zero. In the picture attached below the blue colour curve starts from a negative...

09 February 2021 4,642 3 View

Why there is need to calculate the chlorophyll content and chlorophyll index. What protocol should I used for calculating chlorphyll in mustard?

I want to know the best and effective method/ protocol to calculate the chlorophyll from mustard plants. Please suggest some relevant and the methods or protocols from which I can easily calculate...

05 January 2021 9,882 3 View

Which staining method is best for Archaea?

Generally to differentiate between bacteria we use gram staining technique, broadly based on the composition of their cell wall. However archaebacteria have different cell wall composition, which...

14 December 2020 9,703 5 View

Can a subclinical case of a rare syndrome be published as a Clinical diagnosis?

This case is important from a clinical point of view. Karyotype was normal. Advanced genetic tests were not conducted due to unavailability of such tests in the state as well as financial concerns.

07 October 2020 398 3 View

Any travel grand available to attend conference in abroad?

Suggest All, If any travel grand available for an independent researcher to present in conference i.e. American Society for Microbiology (ASM), USA

04 March 2020 5,629 2 View

For RNA isolation ,adding trizol directly into the culture flask (T25) will harm the cells?

Adding trizol directly into cell culture flask will damage the cells and what is the other alternative to add trizol into cells?

20 November 2019 5,316 2 View

How can we choose our datasets for analysing big data in Healthcare Domain using deep learning Techniques?Reliable dataset?

In the area of Big data Analytics and Deep Learning.

18 December 2018 6,344 5 View

Why do SPSS excludes an Independent variable in the linear regression analysis?

I am working on the project "Effect of dividend policy on firm's vale in India", my project is based on the study of one Nairobi student. Following are regression equation variables Value of...

09 November 2018 8,851 3 View

How to calculate siRNA concentration for Neon transfection ?

Hello, I am using Neon transfection system to transfect siRNA into suspension cell line. When calculating siRNA conc., does it have to based on tip volume or final volume. Although, i have an...

18 July 2018 5,766 2 View

I have loaded hydrophillic drug on uncoated iron oxide nanoparticles, loading is ok but drug release is very slow? Why?

Is there any chances of coating of drug? or May at low pH my drug will release?

24 March 2018 4,827 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Sutter electrode holder leaking after applying positive pressure. How to fix?

I am using a Sutter electrode holder for my patch clamp experiments, but I'm having trouble keeping positive pressure inside my electrode due to a leak out of the back of the holder. I have added...

02 March 2021 5,829 1 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View