Is there anyone who has worked with B16-F10-LUC2 cell line?

Seyedeh Asma Najibi @Seyedeh-Asma-Najibi-2

16 March 2023 1 7K Report

It is the first time I want to grow this cell line. I thawed the cry-tube which was in nitrogen tank and then try to aspirate it with proper medium.The cells was not aspirated completely. Can you help me please?

Seyedeh Asma Najibi

Hello,

Hope you are fine. You can aspirate your cell suspension for 4 or 5 times(with moderate rate). The cell suspension should be homogenous. If Some small particles remain, It's fine. You can use this suspension for growing cells.

Best,

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