Is there anyone familiarized with clouds in biomedical research? Or any publication I could review for guidance?

More Claudia Machicado's questions See All

Does anyone know a source for theta-replicating Staphylococcus plasmids that are compatible?

I am trying to put 3 separate plasmids into Staphylococcus aureus and I am looking for those that are theta replicating and compatible. I have obtained pSK9065 (from pSK1) from Addgene, but the...

23 July 2024 6,968 0 View

How to maintain intestinal tissue resident cells (TRM) in culture?

I am attempting to culture tissue resident T cells (TRM) derived from fresh intestinal tissue for up to six days. I am finding that the viability of the T cells is low at D0, and a very small...

08 July 2024 4,887 2 View

Help with in vitro testing of candida biofilm susceptibility?

Hi all, Would anyone have any suggestions for articles and protocols for performing microdilution tests on candida biofilm? Do you recommend diluting the drug in the medium and adding it to the...

19 June 2024 4,420 3 View

How to reconstitute ATCC of B.ovatus?

I am trying to reconstitute the bacterium B.ovatus ATCC. it was first placed in a THIO broth and despite this we observed no turbidity. This was done 24h after the stick containing B.ovatus ATCC...

12 June 2024 1,644 3 View

Someone can help me with error prone PCR?

How can you regulate the number of random inserted mutations in error prone PCR?

14 May 2024 673 2 View

How do I mention translation in an IPA research project?

Good evening, My IPA (Interpretative Phenomenological Analysis) research project involves conducting interviews in a different country and in a different language. Considering that I will need to...

05 May 2024 6,147 2 View

False Discovery Rate -- how many p values do you correct?

I am using this code (below) to use FDR to correct p values because I ran three exploratory growth curve models. How many p values do you need to correct? Do I just correct all of the p values for...

02 May 2024 4,028 4 View

Help to detect aggrecan in WB, anyone has detected the 250KDa band in mouse brain homogenates?

We are interested in detecting aggrecan (250 KDa) by WB. No luck so far, are there any recommendations, such as running buffer, sample loading buffer? Thank you in advance!

05 April 2024 6,269 4 View

How design Specific Primers for Reverse Transcription?

Hi :) How should I design primers for (-)ssRNA samples from Influenza A and hRSV viruses? I need to select the protein, locate the gene sequence, and then use it to design primers. Should I...

04 April 2024 4,896 1 View

Can I use feces preserved in formaldehyde to perform the McMaster technique?

Can I use feces preserved in formaldehyde to perform the McMaster technique for Haemonchus research?

20 March 2024 1,339 0 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Handling Missing Data and Building a Predictive Model with Incomplete Information ?

I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...

10 August 2024 4,005 2 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

May members post flyers about opportunities to present at a conference? If so, where to post?

May members post flyers about opportunities to present at a conferehttps://veraeducation.com/nce? If so, where to post for the Virginia Educational Research Association? https://veraeducation.com/

08 August 2024 4,585 1 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

Jeff Sedayao

Gene sequencing is one biomedical area that uses big data and cloud computing. I have included some links below.

https://bmcbioinformatics.biomedcentral.com/articles/10.1186/1471-2105-11-259

http://www.biomedcentral.com/content/pdf/gb-2009-10-11-r134.pdf&embedded=true

Claudia Machicado

Thank you Jeff, very interesting!

Stephen Gliske

The iEEG portal (ieeg.org) is an example of cloud storage for EEG data. Also, cloud storage is implicit in several of the NIH "Big Data to Knowledge" "Centers of Excellence for Big Data Computing." The details often depend on the type of data you have, and the type of access and processing you plan to do.