I will like to check the BBB structure on a stroke model of rat. However, I cannot spend too many sections for BBB. Can anybody provide me with some suggestions on how to look at the BBB structure by doing some histology work only?
In rats the best antibody to study BBB integrity is EBA (Endothelial Barrier Antigen), that is negative when BBB failure occur. You can combine it with Glucose transporter-1, that is the best preserved one. You can have a look to one of our papers on it.
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