Is there any windows based software which can calculate Position Weight Matrix (PWM) from align fasta file?
You can use R or Python for this. You may check the following links
https://www.biostars.org/p/166472/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3394284/
https://bioconductor.org/packages/devel/bioc/manuals/TFBSTools/man/TFBSTools.pdf
http://dambe.bio.uottawa.ca/software_download/LabManualDAMBE.pdf
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