How to extract peak coordinates from a given cluster with MATLAB as in the SPM results viewer?

Hello everyone, I am trying to extract peak coordinates from given clusters in an 3D fMRI group image, e.g. a thresholded ICA z-score map. Using MATLAB/SPM I am able to extract a single peak...

26 January 2021 7,672 2 View

Is there any index number for first author in journal article like h index?

In every research paper, first author contribution is comparatively high. To tribute their work is there any index number for an author acting as first author in different papers?

15 December 2020 8,231 14 View

How to identify original journal website?

There are two websites for few journal with same journal title and same ISSN number. For example, There are two websites for the journal titled "High technology letters (ISSN: 1006-6748...

18 November 2020 7,650 6 View

Northern blotting advice?

Hi everyone! Does anybody know about a good website or place to get some guidance on Northern blotting? Also, advice on blotting the RNA ladder?

07 October 2020 739 3 View

Appropriate stats test for multiple DV, multiple treatments, paired across days?

Hi all, I'm trying to determine the right stats test for the project I'm working on. The setup is: 3 different treatment groups e.g. A, B, and C The same treatment is applied across different...

03 July 2020 4,487 7 View

Should there be sex education in schools and institutes about female menstruation?

02 July 2020 5,724 13 View

Do you have a specific question in glaucoma that may need a systematic review and/or a meta-analysis?

I have ophthalmology resident trainees who want to perform a systematic review and/or meta-analysis in glaucoma and want opinion from other experts about what topic seems relevant for them in this...

21 May 2020 6,692 1 View

Is there any correlation to relate temperature and shear strength of Aluminum alloy AA2024-T4?

As shear strength of AA2024-T4 alloy is inversely proportional to the temperature. is there any equation to predict shear strength of AA2024-T4 with respect to temperature?

12 May 2020 8,804 2 View

What is the protein composition of complete Freund's adjuvant?

The complete form of this adjuvant uses heat inactivated and dried mycobacteria. However, to rule out cross-reactivity with an antibody, I would like to know which proteins are left in the...

03 May 2020 1,332 2 View

What do you think about journal taking more than a year to publish a paper?

In this modern world technologies are updating too fast. In this scenario many journals take more than a year to publish a research article which may be an obsolete technology/process.

27 April 2020 9,394 11 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View