I'm new in the area of plant secondary metabolism. So i wanted to know if there is any way to genetically express some specific secondary metabolites (Alkaloids, Terpenes, etc) on diferent microorganisms (Bacteria, yeasts, microalgae).
Yes you can use against microbes against disease causing bacteria if it give activity then u can check on animal model so, you can get idea about its toxicity.
Yes, please refer to the attached paper for using microbial platform for plant isoquinoline alkaloids (IQA) production.
Title: Microbial production of isoquinoline alkaloids as plant secondary metabolites based on metabolic engineering research
i saw one study about vanilla. they take vanillin gennes from vanilla plant to production vanillin by bacteria. also, there is rebodiosides. some GDO yiest and bacteria secreting ribodioside a and b agains to stevia.
Attached is a Review paper (2015) titled "Engineering of Secondary Metabolism". In the paper, it discusses the methods and challenges of using microbes (such as E. Coli) for producing plant secondary metabolites (yellow highlights).
it may useful for you
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4310283/
How can I measure the MIC of different concentration of plant extracts? Is there any easy methods?
@ Mohammed Amzad Hossain,
For evaluation of the Minimum Inhibitory Concentration (MIC) from plant extracts, this attached paper has detail description. Hopefully, it is useful to you.
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