Is there any way to determine abnormalities in the cultured mesenchymal stem cells in vitro? If I would only use karyotyping, there is insufficient evidence to determine the safety of the cell.
Normal cells in culture show contact inhibition (density inhibition) of cell proliferation. Transformed cells lack this property, that is, providing them enough growing factors, i.e. daily changes of medium, they continue growing despite confluence (actually this property is used to test transfection of oncogenes). In conclusion, if cells keep contact inhibition you could assume they aren't transformed (though the use of vertical flow is always advisable).
Thanks Carmen Hoz alot but one more question. if only used karyotyping, there is insufficient evidence to determine the safety of cultured cell in vitro about aspects of genetic?
Tumor cells use to have abnormal karyotypes. As you probably know they tend to be aneuploid, hyperploid, and show aberrant chromosomes. Anyway I think one should work in cell culture under personal safety conditions. A vertical flow hood is a basic requisite of a cell culture laboratory.
Thanks Carmen Hoz a lot. I hpe we'll discuss more in the future. Thanks again.
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