I need to find which transcription factors regulates the expression of a specific gene in mouse/human kidney, is there any array or assay for this kind of purpose?
Hi, Im trying to do an MTT assay with UT7 cells ( in suspension). I start my asaay treating the cells with different EPO concentraions and another inhibitor. There are 4 96 well plates- 0-72...
02 April 2022 9,277 2 View
I have generated a protocol for extracting signal tubular cells form mouse kidneys. The RNA extract is with no protein or phenols contamination and with high concentration. However, the RNA...
12 February 2020 5,791 1 View
when is the peek concentration of Streptozotocin in mouse blood after i.p. injection?
04 January 2017 8,577 2 View
I am looking for a situational empathy questionnaire, (not dispositional empathy) to measure empathy after a manipulation that I hypothesize that will increase empathy.
22 July 2013 8,987 14 View
I would like to know if you are familiar with the work of mirroring in dance movement therapy (DMT) (Mc Garry &, Russo, 2011), that has shown to activate the Mirror neuron system, and to...
26 May 2013 2,324 1 View
need to review articles on care giving in dementia and challenges faced
12 August 2024 6,424 1 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...
04 August 2024 6,265 1 View
On a personal note, even though technology has attracted a lot of interest and funding to combat climate change, it is becoming more and more clear that taking care of the "Human Dimension" is...
02 August 2024 6,773 12 View
I am conducting my research regarding blood coagulation in patients with CKD. I observed a reduction in post hemodialysis aPTT and PT values compared to pre hemodialysis values. But most of...
30 July 2024 3,467 0 View
I'm a PhD Student. From Northwest Agriculture and Forestry University China. My Problem is this I start my work on bhlh79 transcription factor gene. I do my Y2H 5 time but the colonies appear on...
30 July 2024 1,412 8 View
Dear colleagues and scientists: I am asking for your help and insights to develop and explore the feasibility of using an immortalized kidney cell line, for example, HEK293T, for the induction of...
29 July 2024 5,622 3 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
Hi, I will run an experiment where reaction time will be the dependent variable, tested in 2 conditions (with the same participants). I want to control for a confounding variable (individual...
20 July 2024 1,943 1 View