Hi Mahmod,

An easy way to quantify biofilm production of environmental isolates is a microtiterplate assay using kristalviolet. More information regarding the procedure can be found in Bosmans et al. 2015.

For a more reliable estimate in realistic conditions, we use a pilot-scale system to evaluate biofilm formation capacity (and efficacy of chemical or physical treatments to remove biofilms). Fot the latter: see Vankerckhoven et al. 2011; Bosmans et al. 2016; and Lambert et al.