Platelet concentrate are popularly used as PRP or PRF. Though platelets can be counted in PRP and validated whether one really has got a sufficient concentration or its just placebo. I really did find any technique to verify the same in case of PRF, due to the basic fact that it clots and the platelets gets clumped up. Anybody can suggest any alternative technique? i want to do a short study related to the same.
Perhaps microscopy? You might be able to 'smear' some of the clotted material (or maybe smash it), and add a platelet-reactive stain. What comes to mind is fluorescence microscopy, where you can add a fluorescent antibody (anti-CD62P for example). Or maybe see if you can treat the clot material as if it was a solid tissue biopsy, and have someone that does paraffin tissue mounting help you make actual 'tissue' slides which you can then stain with any number of antibodies.
Thank you Craig Sir, will try smearing and paraffin block both and get back to you. Thankyou so much for the tips.
I suggest you try using an aggregometer or if you can't get one, use the OD - optical density of the PRF, compared with that of a standard (PRF with known platelet count). Hope this helps.
- Badges
- Science topic
- Similar topics
- Chemistry
- Pharmacology
- Medical Pharmacology
- Clinical Pharmacology