Is there any technique, method, or instrument available to simplify the counting of orchid seeds, which is currently a challenging task due to their minute spore like size and large quantity of plates, making it impossible to achieve accurate results with the naked eye or under a microscope, given the direct connection between orchid germination and seed counting?
Hi Tshering lhamu Sherpa,
Donald Kirby and colleagues counted orchid seeds as follows: "During processing, we cleaned seeds of debris and subsequently weighed, counted, and examined them for presence of embryos using a dissecting microscope at 30X. We placed a grid on the bottom of a petri dish to facilitate examination and accurate counting; we used mechanical counters to increase accuracy."
(Title: Seed Production and Maturation of the Western Prairie Fringed Orchid. The Prairie Naturalist, 42(1/2), 2010)
I don't know how helpful this article is, but Kirby, the corresponding author could be asked for further details.
"Thank you so much for your prompt reply! I truly appreciate your help. I will definitely give the method you suggested a try. Your guidance means a lot to me, and I'm grateful for your assistance. If you have any additional resources or links related to the topic, I would greatly appreciate it if you could share them with me. Thank you again!"
During a project on thousand seed weight (TSW) of species from the flora of Hungary, we counted and measured TSW of two orchids among other monocots. The title of the paper:
THOUSAND SEED WEIGHT RECORDS OF SPECIES FROM THE FLORA OF HUNGARY, I. MONOCOTYLEDONOPSIDA. I don't know if it is interesting for you or not, however, the link of the paper is here:
Article Thousand seed weight records of species from the flora of Hu...
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