I want to investigate the effect of some nanomaterials on the plants using in vitro culture. So it's important to make solution of those nanomaterials to use in MS media with different concentration. The nanomaterials I want to use are MgFe3O4 and MoO3.
The suitable method to use is to first dissolve your nano material in sterile water, mix thoroughly preferably through sonication and then make your different concentration using the tissue culture media.
Required amount of Nanoparticles you can simply add to your culture media, or you can make stock solutions of Nanoparticles in sterile water and dilute according to your need to add in your culture media. In each case ensure that your nanoparticles are throughly dispersed. Ultrasinication is good option for mixing properly. In some cases Nanoparticles unique characters may get degraded due to heat, so preferably donot autoclave the nanoparticle solution. Add it after autoclaving the MS media. Nanoparticle solutions can be filter sterilized separately.
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