I need to be on road for 4 days from my lab to TEM lab, therefor I need appropriate media to transport my phages. I dropped 3-4 plaques on 400 µl SM buffer and added 20µl chloroform to it. Could it conserve structure of phage for TEM micrograph?
Phages are quit stable so 4 days travelling will not have much effect on it. Just use your original phage lysate having titre upto 10^-5 or 10^-6. if your phages are chloroform sensitive then don't add chloroform it might affect the structure of your phage. Prior to TEM centrifuge the lysate and then use the supernatant for further process.
But to be on safe side if you want add 2-3 drops of chloroform (10%v/v) to 2 ml phage lysate.
Best regards for your guidance.
I tested cacl2 for transport Siphoviridae phage without chloroform and it worked very well. My phage were alive and had appropriate micrograph. Also, for Podoviridae phage Mg2so4 without chloroform were useful.
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