11 Questions 11 Answers 0 Followers
Questions related from Reza Azizian
Hi Dear Friends I need T4 Phage DNA for cloning of Recombinase enzymes. I would be pleased if someone could inform me how Can I get it free for my thesis. Best regards Email:...
13 January 2024 2,198 0 View
I tried to set up RPA with gp32, bst taq polymers and uvsX but without uvsY, Ck and PC. But I don't have any band in gel electrophoresis. I cleaned up RPA amplification by PCR clean-up kit. But...
25 September 2023 4,108 0 View
Is uvsY optional in RPA method?? What about PEG?
27 June 2023 5,525 0 View
For RPA assay I need to use recA instead of gp32. Is it work?
25 June 2023 2,503 1 View
There is a need to have ATCC or DNA of Mycoplasma pneumoniae, Streptococcus pneumoniae, and Moraxella catarrhalis. I would be pleased If I could have your help. Best...
20 June 2022 6,577 3 View
I need to be on road for 4 days from my lab to TEM lab, therefor I need appropriate media to transport my phages. I dropped 3-4 plaques on 400 µl SM buffer and added 20µl chloroform to it. Could...
01 May 2014 7,834 2 View
I recruited PEG 8000 (10%) to centrifuge my phage which was incubated for 24h at 4c with PEG, and by 35000g for 50 min. Finally, I had a brown-yellowish thin pellet. It was so low that is was not...
22 August 2013 6,810 36 View
I have 6 primers for RAPD-PCR. Can I add all of the primers at the same time to the master mix? Or is it necessary to do PCR separately for each of the primers?
15 August 2013 2,075 7 View
I precipitate phage by PEG6000 (20%) at 8500 rpm, 4c for 20 min.
03 July 2013 1,489 4 View
I stored my phage in 20% Glycerol at -20'c. After 2 months it still had function but after 4 months it lost its function and was not able to produce a plaque on a plate. Also, its titer for...
23 February 2013 833 3 View
As an experiment, serial dilution of phages were utilized to prevent biofilm formation, and there is a awesome result. Pure phage and 1/10 dilution have no strong effect whereas 1/100 and 1/1000...
10 January 2013 1,677 1 View
