13 Questions 15 Answers 0 Followers
Questions related from Reza Azizian
Hi Dear Friends I need T4 Phage DNA for cloning of Recombinase enzymes. I would be pleased if someone could inform me how Can I get it free for my thesis. Best regards Email:...
13 January 2024 2,273 0 View
I tried to set up RPA with gp32, bst taq polymers and uvsX but without uvsY, Ck and PC. But I don't have any band in gel electrophoresis. I cleaned up RPA amplification by PCR clean-up kit. But...
25 September 2023 4,158 0 View
Is uvsY optional in RPA method?? What about PEG?
27 June 2023 5,568 0 View
For RPA assay I need to use recA instead of gp32. Is it work?
25 June 2023 2,542 1 View
There is a need to have ATCC or DNA of Mycoplasma pneumoniae, Streptococcus pneumoniae, and Moraxella catarrhalis. I would be pleased If I could have your help. Best...
20 June 2022 6,635 3 View
My phage belonged to P.aerugionsa PAO and was isolated from sewage. Pure phage picked up from middle of plaque and dropped into 200 microliter Cacl2 and 30 microliter chloroform. Phage transferred...
20 May 2014 9,693 6 View
I need to be on road for 4 days from my lab to TEM lab, therefor I need appropriate media to transport my phages. I dropped 3-4 plaques on 400 µl SM buffer and added 20µl chloroform to it. Could...
01 May 2014 7,875 2 View
I isolated phage against P.aeruginosa and tried to set up RAPD-PCR to detect my phages but my primers did not work. Is there a conservative sequence or housekeeping gene that could be recruited as...
21 November 2013 4,224 1 View
I recruited PEG 8000 (10%) to centrifuge my phage which was incubated for 24h at 4c with PEG, and by 35000g for 50 min. Finally, I had a brown-yellowish thin pellet. It was so low that is was not...
22 August 2013 6,851 36 View
I have 6 primers for RAPD-PCR. Can I add all of the primers at the same time to the master mix? Or is it necessary to do PCR separately for each of the primers?
15 August 2013 2,116 7 View
I precipitate phage by PEG6000 (20%) at 8500 rpm, 4c for 20 min.
03 July 2013 1,529 4 View
I stored my phage in 20% Glycerol at -20'c. After 2 months it still had function but after 4 months it lost its function and was not able to produce a plaque on a plate. Also, its titer for...
23 February 2013 876 3 View
As an experiment, serial dilution of phages were utilized to prevent biofilm formation, and there is a awesome result. Pure phage and 1/10 dilution have no strong effect whereas 1/100 and 1/1000...
10 January 2013 1,718 1 View
