In most of the papers to perform a pathogenicity test the fungal inocula has been used in a concentration of 106 or 107 conidia/ml. is there any standard behind this?
Please see the attached publication which may be useful. In general, higher population of inocula is used to overcome the resistent from native population.
Dear Trepti Singh,
The level of inoculum to produce a single lesion is a minimum for accomplishing a pathogenicity test is the inoculum threshold.
As inoculum level increases the number of lesions and severity of the disease will increase.
This testing usually needs about exponent 6 or 7 for saturation the higher level will not necessarily be useful and at very high levels the resistance of plants can be overcome.
Ideally the levels of inoculum can be tested in pathogenicity tests to give a standard curve and you can select a level for your testing based on this test.
Otherwise many researchers get good elucidation of disease response at exp 6 or 7 and holding plants in a mist chamber for 48 to 72 hours before being put into a greenhouse bench. This works quite well for most fungal foliar pathogens.
Best wishes on your tests.
Thnks a lot sir.
Could you please provide me any reference to perform the test to select the level of inoculam
Hi: Most literatures for the pathogenicity test use 10^4 spore suspension.
Hi Trepti
The concentration of spore suspension depends on type of fungal pathogens, but the literature indicated to use the concentration 10^5 spore suspension
1. Check the literatüre first.
2. The higher the inoculum the most destructive the disease.
3. Depending on the type of the resistance, you either increase or reduce the amount of incoulum.
4. The time in the year and the infection production type ( artifical or natura) are important as well.
Good luck.
Nusret
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