Hi...
Is there any standard technique for culturing endophytes? Different articles stated different sterilizing techniques using different concentrations of disinfectants for different periods of time. This is confusing that which surface sterilization technique is most effective.
Secondly, for culturing endophytic bacteria and fungi, many articles stated to put plant tissues on to the culture media plate after their surface sterilization done. But this is not mentioned that is it necessary to expose tissue's inner surface on the media or culturing their exterior surface is enough to get pure growth of endophytes only?
Please answer to these queries.
Regards