Hi all,

I am currently planning to make the allergy model mice by injecting OVA and aluminum hydroxide (as adjuvant) dissolved in saline.

However, reading papers, it seems like the amount of antigen and their solvent (PBS, saline, etc.) vary widely from one research to another. I am not sure which and how much of these I should use for inducing allergic reactions in mice, and I would be grateful if anyone let me know how to make a standard OVA-induced allergy mice model.