Researchers usually simulate allelochemicals release pathways ( volatilization, rain and fog leaching, root exudate, residue decomposition ) through different allelochemical extraction methods. The most commonly used method is the extract method. Bioassay experiments were used to investigate the effects of extracts on germination, seedling growth, and physiological processes in receptor plants. However, the extraction solvent, concentration, receptor species, observation methods and monitoring indicators used for the experiment by different researchers are very different, making it difficult to compare the allelopathic results between studies and the allelopathic potential differences between plants. Is there a standard protocol for assessing plant allelopathic potential?

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