What can I do for the re-isolation of phages and separation of mixed DNA of bacteriophages?

Dear researchers During my thesis isolation of phage and also re-isolation from one plaque and passaging of that to reach a high concentration, I extracted the DNA and catch TEM. My TEM images...

09 June 2020 1,747 6 View

How to conductive polyethersulfone membrane?

Please suggest a way to conduct low voltage poly ether sulfon membrane.

24 December 2019 5,108 3 View

Is it possible to use Hydrochloric acid to regulate the pH for MES buffer?

I have the salt for making the MES buffer ( 2-(N-morpholino)ethanesulfonic acid ) and 0.1M solution of it give me a PH of almost 7 which I have found out it's due to the DI water( I used to make...

25 December 2017 3,338 2 View

I need a SAS procedure

How i can compare means in one way anova and two way anova for data that are normal but variance of homogeneity assumption is not held in SAS software

13 June 2017 1,722 2 View

How is chimera detection for 18S rDNA sequence carried out?

I want chimerism checking for eukaryotic organisms (worms); please let me know if there is software or database. Is the Chimera detection for eukaryotic organisms are used?

03 June 2017 572 2 View

Can anyone tell me, what is the end group of this co-polymer?

24 September 2016 4,738 3 View

How can Liquid crystal elastomers (lyotropic) show liquid crystal phase in a solvent ?

04 February 2016 6,495 3 View

How can calculate the band gap energy of crystalline and amorphous metal oxide from UV-VIS spectroscopy?

05 January 2016 7,903 4 View

Does anyone have experience with SureSelect XT2 exon capture?

I am working with SureSelect XT2 all exons-library prep protocol and I am getting different yields every time I am using this protocol. I am a little uncertain about the first Ampure clean up step...

17 June 2015 8,465 1 View

What is the temprature of calcination and sintering of gama and amorphous alumina shell without changing in phase of shell?

I am trying to synthesis alumina-gama alumina core/shell.

09 January 2015 8,090 1 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

Do anyone here can help us in the field of molecular docking?

We have synthesized various metal complexes with different environment around the metals. Now to see the application of synthesized complexes we need this study also. we are also interested in...

01 March 2021 1,899 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

Which transition metal salt precursor is best for doping? Why?

I would like to synthesize a transition metal doped TiO2 via solgel method. Hence, I would like to know which metal salt precursor to choose among Chloride, Nitrate, and Sulphate. A clear...

27 February 2021 4,285 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

How can we theoretically calculate Elemental analysis(percentage) of atoms in covalent organic framework?

For example in one research article the Elemental analysis values of COF-TpPa-1 ( Triformylphloroglucinol (Tp) (63 mg, 0.3 mmol), Paraphenylenediamine (Pa-1) (48 mg, 0.45 mmol) are as follows...

24 February 2021 1,265 6 View