31 July 2016 4 3K Report

I would like to prepare small unilamellar vesicles using probe sonicator. Therefore, I am intended to buy one probe sonicator. Can anyone tell me which sonicator to buy (Specification)?

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What is the best way to fix plasma derived miRNA for subsequent cDNA synthesis?

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03 February 2021 9,330 3 View

What is the general miRNA yield one gets from the Qiagen miRNEASY serum/plasma advanced kit?

I am using the Qiagen miRNEASY serum/plasma advanced kit, using 400ul of frozen plasma. What is the yield you get on average? I am using nanodrop currently for quantification. I am getting...

02 February 2021 8,806 2 View

What dilution do I use for my GNP solution UV-Vis and DLS characterization?

I have prepared gold nanoparticles using the citrate reduction method in the lab. I need to characterize them using UV-Vis and DLS, by how much should I dilute my sample for using it in the...

26 January 2021 530 5 View

What should be the ideal Thiolated-Oligo:GNP ratio for effective conjugation?

I need to conjugate thiolated oligos to GNPs and I am wondering what concentrations of the oligo to use with what concentration of GNPs for effective conjugation.

08 December 2020 7,975 2 View

What methods can I use to confirm if my oligo-GNP conjugate is hybridizing with my target nucleic acid?

Once I have oligo-GNP conjugates, I need to use them as sensor for my target nucleic acid. Before doing that I need to confirm whether the oligo probe (conjugated to GNP) hybridizes well with my...

04 December 2020 4,734 2 View

I need to design primers for hybridization chain reaction for signal amplification (in-vitro). Anybody know a software/company that does this?

Hybridization chain reaction (HCR), a form of non-enzymatic method for amplification of target nucleic acids. I need to design the two HCR primers. Is there any special software for doing that?

27 August 2020 1,008 1 View

Suggestion for buying speed vaccum table top for conentrating samples ?

I need a suggestion for buying a speed vac system for drying solvents/concentrating samples after SPE.

24 August 2020 648 2 View

Can we design aptamers that target other nucleic acids?

Aptamers in most cases are themselves nucleic acids, generally used to target other bio-molecules. I wonder if we can design aptamers in such a way that they target other nucleic acids. Will...

06 June 2020 3,132 1 View

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