Is there any specific power requirement of probe sonicator for preparing small unilamellar vesicles ?

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Tripta Bhatia

Hi,

For SUVs preparation, I have used a tip-ultrasonicator (Misonixs 3000, Qsonica, Newtown, CT operating at frequency 20 kHz). You can download the manual here with specifications. http://www.sonicator.com/literature/manuals/Sonicator3000Manual.pdf

The protocol for SUVs preparation can be found in Biochimica et Biophysica Acta (BBA) - Biomembranes Volume 1848, Issue 12, December 2015, Pages 3175–3180.

Regards from Tripta

Irina Panteleeva

An alternative to a probe sonicator is a Bioruptor https://www.diagenode.com/p/bioruptor-plus-sonication-device

Mauricio Baptista

Any tip sonicator with power above 150W will work well to sonicate aqueous suspensions of lipids (formed by hydrating a lipid film) and transform it in a pale-blue transparant aqueous suspenstion containing SUVs. The concentration of 5-10mg/ml of lipids is ideal.