I would like to use the specific marker to identify secondary granule and tertiary granule in zebrafish neutrophil. Do you have any suggestions? thank you!
A typical reforming section in a large-scale Haber-Bosch process (Figure 1) comprises two reactors. A mixture of methane (CH4) and steam (H2O) is fed into a primary reformer to produce syngas via...
01 August 2024 7,051 5 View
There is an error message "interface silicon/oxide not found in grid xxx.tdr file"
26 June 2024 5,551 0 View
Dear all, I want to calculate the phonon dispersion of 4CNR-1-0 (structure is in Fig. 2 (b) in this article Designing coved graphene nanoribbons with charge carrier mob... ) and then calculate...
17 June 2024 6,911 2 View
Monomer: NIPAM; Initiator: BiBB; Catalyst: CuBr; Ligand: PMDETA I added CuBr and the mixture was blue, after that, I purged with nitrogen for 30 minutes and the mixture turned green. The mixture...
27 May 2024 9,144 1 View
CIR 2021/808 written as "the CCα shall be calculated as follows: Method 1: by the calibration curve procedure according to ISO 11843-1:1997 ( 14) (here referred to as critical value of the net...
13 May 2024 9,481 0 View
Electrochemistry is a pathway to convert CO2 into valuable products such as CO. However, a potential side product in the cathode is H2. I wonder if it is possible to tune the composition of the...
12 May 2024 9,061 3 View
We bought the E-beam evaporation system without a loading chamber. until now, we have used this system about 20~30 times for the deposition of Al2O3, Ti, Au, and Al having thicknesses from 50~100...
23 April 2024 5,156 4 View
Hello everyone, I am working on a project where I receive daily samples to freeze at -80 °C. Specifically they are peripheral blood mononuclear cell (PBMC), until now I was using CryoStor® CS10,...
22 April 2024 3,905 6 View
I've recently been trying to investigate the inhibition of H2S-synthesising enzymes: CBS, CSE and 3-MST, via PCR. We've ordered EGCG, PAG and i-3MT-3 to inhibit each enzyme respectively, and have...
16 March 2024 392 1 View
Pulsed electric field is considered a "non-thermal" method for food processing that involves the application of short high-voltage pulses to create pores in cell membranes. However, since I am not...
13 March 2024 2,624 6 View
Dear all, We are producing activated carbon from hazelnut shells but have an issue about the dust of the a.c. granules also known as the dust film on granules. Linear vibrating seives doesn't do...
11 July 2024 9,782 2 View
Hi, scholars. I am planning to conduct mouse neutrophil and T cell co-culture in Vitro and observe the effect of neutrophils on T cells. I don't have any protocol. Could anyone provide me...
19 June 2024 3,271 2 View
Hello! I am planning on doing co-incubation experiments of neutrophils with exosomes and wanted to ask what concentration of exosomes, cell number and incubation time works with this cell...
29 May 2024 6,551 0 View
I recently discovered that BFA is used in flow cytometry, enabling the observation of intracellular cytokines that would be otherwise released. Is the same principle applicable to ROS? I would...
04 March 2024 7,094 1 View
Hi, I am using 2 gradients Percoll protocol to isolate neutrophils from whole blood. I prepare gradients of desired density myself on the day of the experiment from Percoll (Sigma 1644). In my...
28 February 2024 9,499 1 View
How to count neutrophil
26 February 2024 5,068 3 View
HemInozoin cristals are very compact in human parasites. However in opther haemoparasites as order haemosporidia is observed volutin. Do you think is there any correlation between protein...
26 February 2024 3,806 2 View
I have used 10% FBS and this image i took after 3 days of passage. and I don't see any cells floating from where i think it might not be contamination but I don't understand what it is. If this is...
18 February 2024 9,843 3 View
Hello! I am experiencing some problems in detecting neutrophils in rat blood of F344 rats. I was using RP1 as a marker but somehow the detection fails. I was wondering if there was a good...
04 February 2024 1,918 0 View
Dear all, Hello, I am recently working on isolating neutrophils from murine bone marrow by using Histopaque 1099 and histopaque 1077, but the purity of the cells are always unsatisfactory. Here I...
01 February 2024 9,203 3 View