I would like to use the specific marker to identify secondary granule and tertiary granule in zebrafish neutrophil. Do you have any suggestions? thank you!
Hi everyone. I bumped into the difficulty that I couldn't get any signals from my brain section in the DG region of hippocampus. However, I have standardized the BrdU protocol in my brain...
13 January 2021 9,449 0 View
I added two times the same thing by mistake and I need to remove one. Thanks.
11 January 2021 6,118 5 View
Hello, I tried to perform a co-simulation between PScad and SIMULINK. I reviewed the requirements of compatibility between them but I still have no clue about this issue. My PScad is 4.6.3 (x64),...
06 January 2021 8,226 1 View
Do you know any journals in the area of medicine/pediatrics/nutrition that publish protocols of systematic reviews? No APC or Low APC
04 January 2021 292 14 View
I am currently working with S. oleraceus and I would like to know if there is a practical method to accurately counting the seeds other than weighing them or simply counting one by one? Thank you...
02 December 2020 6,988 3 View
Hi! So I'm currently studying the diversity difference between 2 ecosystems. I ran a Shannon-Wiener test on site 1 and site 2 and found the indices of 1.525 and 2.165 , respectively. I have done...
22 November 2020 6,798 1 View
Dear colleagues, In order to evaluate the performance of concrete slabs-on-grade, the sub-grade should also be considered in the testing setup. Using soil or sand to reproduce the sub-grade is a...
20 November 2020 7,921 1 View
Hello everyone! I have a problem with when the determination of available silicon in fertilizer by UV-Vis. I do that following method A 5-Day Method for Determination of Soluble Silicon...
10 November 2020 1,370 10 View
Please see the attachment. In my case, I tried a lot of time on the Nissl staining on 10um cryosection. I am not sure which step goes wrong? I make sure my anti-rolling glass is intact. seem like...
07 November 2020 9,568 3 View
Hello everyone. I would like to compute the dynamic hyperpolarizability value of a molecule using Gaussian on Linux OS. I have read that the essential keywords to include for such request are the...
07 November 2020 5,620 4 View
Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...
03 March 2021 1,499 2 View
I need to make an injection molding machine in which short fibers are to be introduced. Short fiber length and material properties are to be determined and also the material strength are to be...
22 February 2021 4,290 1 View
Greetings, The examination of the interaction zone between Fusarium oxysporum and Pseudomonas by light microscope revealed clear hyphae without septa and the presence of granulations (vesicles),...
05 February 2021 5,523 4 View
I am trying to visualize alpha and dense granules in platelets. However, it seems like the granules were not stained well. Is there any way I can improve my staining technique to obtain better...
04 February 2021 5,925 9 View
I tried several times extrating RNA from human neutrophils and the amount of RNA at the end of the precedure was minimal. I also tried using colunns from QIAGEN. Never had any problem with tissue...
26 January 2021 8,881 4 View
Hello everyone. I have collected intestinal biopsies from stem cell transplanted patients in RNAlater and wish to quantify neutrophils only via qPCR. As we know that the mucosa of gut is filled...
25 November 2020 2,680 3 View
Hello everyone, I am trying to replicate the process done in the paper 'Shocks near jamming' by Gomez et al (PRL, 2012) where the granules are initially in a pre-stressed state with initial...
21 October 2020 1,210 1 View
I realize that the work flow in most flow-based studies of neutrophils is such that the cells are analyzed without ever becoming adherent to the cell culture surface, ie, as soon after isolation...
14 October 2020 1,579 4 View
I found several papers in the literature where Sytox (green, red, blue, or orange) is used to detect Neutrophil Extracellular Traps, but it's always used with purified neutrophils. Do you know if...
07 October 2020 5,865 2 View
I need to analyze the cross-section surface of highly porous granules with the SEM but I don't know how to prepare the samples. Is there anybody who have an idea of how to flatten porous and...
07 October 2020 4,861 9 View