Why my protein band in SDS-PAGE if heating the sample becomes much lighter compared to not heating the sample?

I ran a SDS-PAGE of my sample ovalbumin in histidine buffer in 2x Laemmli buffer without heating and it got nice visible bands. However, when I heat the sample at 77C in 2x laemmli buffer for 5...

15 February 2021 3,080 3 View

Questions about FTIR and LASER?

13 April 2020 2,513 1 View

Can amino acid change after protease cleavage?

Hello everyone, I am currently working with targeting proteins to yeast mitochondria. Our strategy is to use a mitochondrial targeting signal to transport our protein of interest to mitochondrial...

16 October 2019 6,572 5 View

Mutant mice more like to stay at the center area comparing to control ones in open field test? more anxiety or less?

Hello guys, My mutant mice are trying to stay at the center area comparing to the control ones. Does it mean the mutants are more anxious or other things? thanks!

18 August 2019 4,955 4 View

Why is my whole western blot gray?

I have attached a picture for my most recent western blot. My Beta-actin always shows up completely perfect but all other protein blots appear gray and messy. This has happened multiple times....

31 May 2019 5,364 3 View

Are cells able to preferentially excise/kick out your gene of interest, but not the antibiotic selection gene, from stable cells lines?

Hi all, I am trying to generate some stable cell lines expressing either the WT or mutant version of my gene of interest (GOI). My lentiviral construct consists of a BSD gene for blasticidin...

14 December 2018 9,110 3 View

What is the storage condition of Rupintrivir (PZ0315) from Sigma?

Has anyone used rupintrivir (PZ0315) from Sigma? the rupintrivir powder was recommended to dissolve in DMSO and stored at -20 degree. I am wondering if anyone knows how long the aliquot of...

11 July 2018 9,264 2 View

How to interpret this cell invasion assay result?

I performed a cell invasion assay to asses invasive capability of two different tumor population by using matrigel coated inserts. After experiment, I found that: No. of stained cells on the...

03 January 2018 648 5 View

Does anybody know what type of cell death is this?

After treating a compound to my cells, I noticed that cells died in a way I have never seen before (see the attached image - panel A). I don't think its apoptosis (apoptosis look like panel B)....

13 October 2017 4,349 3 View

Synchronization of Yeast (G2-phase, Nocodazole, SD-trp culture)

26 July 2017 896 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View