Is there any software (besides ENVI-met) capable of thermally analyzing vegetative simulations in urban squares or outdoor areas?

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Is it safe to make a glycerol stock from Agrobacterium transformed cells from a 10-day plate?

Hello all. I transformed some Agrobacterium competent cells by electroporation and performed colony PCR to see the positive transformants. However, I performed the glycerol stock after 10 days of...

26 May 2024 9,304 3 View

Is it safe to purify plasmids of E.coli precultures from 2-month old plates?

Hello all. I purified some plasmids after doing precultures of strains from a 2-month old plate in -4 degrees. The PCR results were fine and I used theses plasmids to transform Agrobacterium...

26 April 2024 8,440 3 View

How has the efficiency of photovoltaic cells improved over time?

On the use of solar energy in homes.

07 April 2024 9,465 2 View

Can someone indicates me a database about meteorological variables per country?

I'm looking for variables like solar irradiation, wind speed, humidity, temperature and precipitation

06 April 2024 1,926 2 View

How to prepare dibenzo[a,h]anthracene stock solution? Which solvent to use?

I have trouble solubilizing the analytical standard of dibenzo[a,h]anthracene (a polycyclic aromatic hydrocarbon purchased from Sigma-Aldrich) to prepare a stock solution. I have already tried...

01 April 2024 5,768 3 View

Mitosox in mitochondrial extract?

Hi everyone, I was wondering if someone has a protocol for using MitoSOX in mitochondrial-enriched extracts. MitoSOX can migrate to the nucleus and generate an inaccurate response about...

24 March 2024 466 0 View

How can I filter kraft lignin?

I have prepared a liquid medium for fermentation with kraft lignin in water, but the lignin was not completely dissolved. After fermentation, I am wondering about the best way to filter out the...

04 January 2024 7,479 1 View

Which stain can be used for visualizing qunencher-tagged DNA oligonucleotide using gel electrophoresis?

I am trying to see a quencher (BHQ)tagged synthetic DNA oligonucleotide on an agarose gel...I used EtBr for the same...I was able to visualize the strands that I have (not tagged by BHQ), but I...

20 December 2023 2,128 0 View

What the best protocol for protein extraction from human neutrophils?

I've tried to use Ripa buffer containing 0.1% SDS + 10% of proteinase inhibitors combined with 3 cycles of freeze/thaw (Dry ice with isopropanol/Dry bath 70ºC), followed by 16.000rcf for 10...

17 December 2023 3,030 1 View

Does anybody know how to make homemade ABI 3500 buffer ?

Ineed a recipe.

13 December 2023 9,782 0 View

How can I find the cracked version of ENVI-met software?

Envi-met software

07 August 2024 8,188 1 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Expansion thermal of muscle ?

What is the thermal expansion coefficient of human muscle?

27 July 2024 7,190 0 View

How can I extract glycosides from mangrove leaf samples without an incubator that can reach temperatures above 60°C?

I want to extract glycosides from my mangrove leaf samples, but my laboratory lacks an incubator capable of reaching temperatures above 60°C. The paper I am following specifies a minimum...

13 July 2024 3,686 0 View

How to introduce on fluent the optical loss of the wall due to the reflection of the plate towards the environment ?

"When solar irradiation reaches a surface, some of it is absorbed while the rest is reflected. Part of the light absorbed by the surface transforms into heat and increases the module's...

13 July 2024 8,621 5 View

Why am I seeing only one initial maximum in torque-time curves for the fusion of PVC in a Brabender?

We're studying the stability and fusion behavior of multiple PVC compounds in a Brabender. After a few trials, we set the temperature at 195 °C and the rotor speed at 70 rpm. Samples differ in...

12 July 2024 2,768 1 View

Is there any materials that can be as flexible and stretchable as PDMS, while also having a small linear thermal expansion coefficient?

For the PDMS material, it's linear thermal expansion coefficient is about 3.2*10-4/℃. The polyimide has a smaller linear thermal expansion coefficient like 2*10-5/℃, but it cannot be stretch. It...

04 July 2024 4,525 1 View

Is there a vegetation zone dividing location based on climate or geographic parameters available by province level?

I am researching the traceability of palm oil and need a parameter that can differentiate between provinces around the world. I want to group the provinces by their vegetation zone and see if it's...

01 July 2024 888 2 View

Thermophysical properties of MWCNTs nanoparticles?

Is there any article can I find all thermophysical properties of MWCNTs nanoparticles especially, thermal expansion coefficient and electrical conductivity?

30 June 2024 4,075 2 View

Is the polymer has low CTE if the polymer has the high chain packing and small free volume?

I wonder the tendency of coefficient of thermal expansion (CTE) of polymer which has different chain structure. In my opinion, CTE will be higher when the polymer materials has high interaction...

30 June 2024 570 5 View