Is there any size limitation for the inserts in bacterial transposon systems?

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I have q question about TOPO TA cloning. I have a PCR Product that Platinum Taq DNA polymerase is used. What should I do?

I didn't design my primers that produce A overhang. And I have to do cloning. I read that I can use dATP and nonproofreading Taq to produce A overhang. Can I use this method to add an A to my PCR...

01 June 2023 3,847 3 View

Plasmid Retention comprarison between Conjugation vs Electroporation ?

Info : Transposon vector does not replicate in the anaerobic gram(-) bacteria that I am working on. This bacteria is sensitive to chloramphenicol. When the plasmid was sent via conjugation,...

30 April 2019 8,000 4 View

We tried to DNA isolation from FFPE tissue by manuel method but we have some problems in PCR. There are so many smear in agorose gel. Help pls?

We isolated DNA from FFPE by manuel method. We done PCR but in gel there are some smear and we dont know how can we solve this problem. Can anyone have a solution?And why saw smear in PCR?

27 May 2018 628 12 View

How can I obtain the required file to estimate k value with STRUCTURE harvester?

Hello, By using the parameter setting below, I am trying to obtain the results for further analysis in Structure harvester. However, even tough I check the option "compute the probability of the...

07 April 2017 10,004 4 View

Which data transformation method do you use when variances are not homogenous?

I have gene expression data which includes negative values. When I run Factorial ANOVA, for some genes, Tests of Homogeneity of Variances (Cochran C, Hartley, Bartlett) suggested a p-value lower...

09 May 2016 4,097 4 View

How can I calculate colony forming unit (cfu) for bacteria??

I want to calculate the colony forming unit of a bacterium which is frozen in glycerol solution. I will test an antibacterial surface so I have to know how many bacteria there are in the LB medium...

08 January 2016 7,670 93 View

How is charge group defined in gromos force field when adding new residue to .rtp file? Is there a gromos bond type for the bond between C and OE ?

I am trying to run MD for fluorescein, a small molecular weight green dye. I need to add a new residue (which exists in pdb file that I created by deleting the rest of the molecule and leaving...

05 March 2015 6,917 2 View

Modified Newcastle-Ottawa Scale for quality assessments of prevalence studies?

Has anyone used the Modified Newcastle-Ottawa Scale for quality assessments of prevalence studies?

01 January 1970 366 5 View

Multiple regression with uncorrelated variable.

Hi, I want to perform multiple regression analysis with 5 IVs and 1 DV. First, I did correlation analysis. Four of my IVs are correlated with DV, but one of them is not correlated with DV in...

01 January 1970 9,784 0 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View