Is there any server to predict binding site of cofactor in a protein?

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Where does phenomenological research fit within the layers or stages of the research onion proposed by Saunders et al. (2018)?

I've selected an interpretivist philosophy along with a qualitative inductive approach for my research. I plan to employ semi-structured interviews as my primary data collection method. However, I...

12 May 2024 7,816 4 View

How can I generate angle restraints using gromacs?

The system that I am working on contains two ligands with a protein and I want to use angle restraints between water molecule and amino acid residue along with the distant restraints.Is there any...

25 September 2020 1,132 0 View

Have you ever got high backgrounds in western blots done with heart tissues ?

Hi, I am doing WB with heart tissues (Rat) these days and having a problem of high background. We have no issue of getting high background for WB done with cells or other tissues, the protocol is...

11 June 2019 2,871 3 View

What are the essential cofactors to perform in vitro assay using alpha/beta hydrolase enzyme ?

Need to perform in vitro reaction with substrates and purified protein (Methyl esterase/alpha/beta hydrolase super family) 1. What are the essential cofactors? 2. Which buffer solution/pH should I...

14 April 2019 9,411 1 View

Measure beta-oxidation of fatty acids?

Hello all, I am looking for a kit or a method to measure beta oxidation of fatty acids (without radio labeling) in tissues (Mouse) and cells (Human). Any suggestions ? Thanks

16 January 2018 8,997 11 View

Getting low fluorescence values for Control group compared to H2O2 group in intracellular ROS detection with DCF-DA probe?

I was trying to detect H2O2 induced intracellular Reactive Oxygen Species (iROS) generation with DCF-DA fluorescent dye (2′,7′-Dichlorofluorescin diacetate, Sigma Aldrich, St. Louis, MO) in HK-2...

09 November 2017 1,675 5 View

Has anyone tried out sekisui diagnostics triglyceride assay kit ?

Has anyone tried out Sekisui diagnostics triglyceride assay kit to determine mouse liver triglycerides ? Though the Sekisui protocol is made for automated systems I'm trying to use it manually....

10 October 2017 3,619 0 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Is artifacts in XPS possible to build high deviation in binding energy larger than 5 eV??

Hello. Thanks for your consideration to see my question. Recently, I conducted XPS anaylsis of g-CN that is prepared from thermal polycondensation of DCDA, so-called conventional bulk-g-CN,...

30 July 2024 9,824 2 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,738 0 View

Does post-translational protein modification cause devisions on observed pI verses calculated pI?

In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....

24 July 2024 8,076 3 View

What is the function of fusion tag GAMGSGIQRPTST?

I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?

23 July 2024 1,353 4 View

Element deletion problem in ABAQUS/Explicit solver using Cohesive elements?

I had a problem for so long. I could easily delete elements in ABAQUS implicit solver but I needed to use VUMAT so I had to have Explicit solution but the element did not disappear for so long. I...

23 July 2024 10,032 0 View