08 June 2022 5 1K Report

I am searching for the main question "why we need to study SPR"

1. Is commercially available SPR devices are not giving enough sensitivity?

2. What is the need of SPR market for improvement?

3. main objective to study SPR

More Tanwin Ashrafi's questions See All
Choosing a statistical test when we have ctegorical moderating.V and two separta group and categorical IV?

In the research, if the independent variable is categorical and the moderating variable is categorical, while the dependent variable is two separate groups. What kind of statistical test should be...

28 October 2023 4,012 2 View

Can anyone help me, how to calculate change in refractive index?

The change of refractive index needs to calculate and match. I can't able to understand how to convert nM to gm/cm3, to make final outcome unitless

10 October 2022 9,975 0 View

If a quantum dot of some elements shows interaction with biomolecules, does same interaction can expect with monolayer of same elements ?

Quantum dots of tungsten disulfide interact with honey. Can monolayer of tungsten disulfide interact with honey?

10 October 2022 384 2 View

Suitable Camera for GRENOUILLE?

Hi there, I hope you are doing well. Do you know, which camera is used recently for the GRENOUILLE technique in order to measure the pulse length of ultra-short pulses? what features the camera...

08 March 2022 9,036 4 View

Why my Hela cell culture is having some round shaped weird structures which obviously are not cells?

Recently I have been observing some strange bloomed like structures as in the attached photo in my Hela cell culture which look so different from my long term experience of culturing Hela cells....

07 September 2021 2,381 3 View

How using linear polarizer increase the resolution of optical imaging systems ?

Hi there, I hope you are doing well. I am working with imaging systems. I am confused about the effects of linear polarizer in such systems ( I mean how a linear polarizer can improve the...

21 December 2019 5,404 6 View

Is there anyone who used C-line analyzer for measuring blood/media glucose and lactate?

C-line glucose and lactate analyzer is quite common in measuring blood glucose and lactate in the clinical studies. I have used it for metabolic study of my cells to find out glucose consumption...

16 March 2019 7,000 0 View

How to present data of glucose consumption and lactate production measurements from cell culture media?

I have some data of media lactate and glucose measured by C-line glucose and lactate analyzer from Biosen. Media were collected from MDA-MB-231 cell culture during and after running some...

24 January 2019 8,715 0 View

What are differences among COLTRIMS and Velocity Map Imaging (VMI) and TOFMS?

Hi there, I hope everything goes well. I want to be familiar with molecular dynamics and Coulomb Explosion Imaging (CEI). Could you please explain what COLTRIMS, VMI and TOFMS do? and in which...

08 November 2018 1,313 1 View

Kerr lens mode lock in femtosecond ti:sapphier lasers!!!?

Hi there, I hope every thing goes well. As we know there are some techniques in generating ultra short pulses such as active mode lock (Acousto-optic / Electro-optic modulator) and passive mode...

03 November 2018 7,548 25 View

Similar questions and discussions
Has the formation of my silver nanoparticles been indicated by the provided UV-Vis spectrum?

I'm trying to produce silver nanoparticles using plant extract, but I didn't observe the expected peak in the UV-Vis spectrum between 380-420 nm, where silver nanoparticles typically appear. After...

04 May 2024 6,463 11 View

Surface mass of a BLI (Bio-layer interferometry; Octet) Measurement?

Hello together, I am looking for a conversion factor for BLI (Bio-layer interferometry) from the nm values into a more comparable pg/mm2? For SPR it seems to be common to convert the RU values...

13 March 2024 6,238 0 View

What could account for the presence of dual surface plasmon resonance peaks in the UV-Vis spectrum of silver nanoparticles?

Respected scholar, I have synthesized bare silver nanoparticles using sodium borohydride reduction at room temperature and measured their UV-Vis absorption spectrum. The spectrum showed two...

26 January 2024 3,191 3 View

ADA confirmatory assay format selection?

I need help with selection ADA confirmatory assay format. Does anyone have the experience of selection confirmatory assay among ELISA, SPR?

14 January 2024 8,923 0 View

How we start programming in matlab for surface plasmon resonance?

SPR

11 December 2023 3,727 3 View

SPR interpretation and troubleshooting?

I tested two analytes with the same a.a sequence, just different by 1a.a to one target protein. My SPR outcome looks like the attached figure below. Can I say the right one binds stronger than...

09 November 2023 6,681 2 View

Can anyone reccommend a GST antibody to tether recombinant proteins to a chip for SPR?

Cytiva sells a GST antibody as part of a rather expensive kit for coupling tagged proteins to their sensor chips. The antibody can not be purchased separately, and we do not need all the other...

26 October 2023 3,800 1 View

What is the reason for the abnormal transmission peak of SPR transmission spectrum?

When I fuse the multi-single-multi fiber structure, coat it with silver film to stimulate the SPR phenomenon, and establish the transmission spectrum, I add water to the sensing area. The spectral...

23 October 2023 6,782 1 View

What is effect of an external electromagnetic field on performance parameter of SPR sensors?

If we place a SPR sensor device between the electromagnet and varying the intensity of electric and magnetic field. How does sensor behaves especially when external electric field and evanescent...

23 October 2023 3,941 2 View

Does the sensorgram of the negative response look like the attached figure? What is causing that?

I have a question: Does the sensorgram of the negative response look like the attached figure? I use a CM5 chip immobilised with His to capture my protein and then use other proteins as my ligand....

12 October 2023 6,255 1 View

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