What are differences among COLTRIMS and Velocity Map Imaging (VMI) and TOFMS?

Hi there, I hope everything goes well. I want to be familiar with molecular dynamics and Coulomb Explosion Imaging (CEI). Could you please explain what COLTRIMS, VMI and TOFMS do? and in which...

08 November 2018 1,272 1 View

Standard error of differences or standard error of mean (Log) in qRT-PCR?

I would like to represent my qRT-PCR results in a bar plot but I am not sure to use SEofdiff or SEM as error bar?

08 July 2018 506 6 View

N of NiO

What is "refractive index (n) " of NiO Vs wavelength?

25 May 2017 4,865 3 View

How could I group my data for analyzing in NormFinder algorithm in NormqPCR package of R?

07 April 2017 8,819 1 View

How can I design primer for heterozygot sequence in real time-PCR?

hello every body i want to design primer for my sequences but because of cross-pollination nature of my plants (Thymus sp) my sequences are heterozygot (they have SNP). I tried to design primer...

15 February 2017 3,653 3 View

How can I find MgO band gap experimentally?

How can I find MgO band gap experimentally? it is a wide band gap material and it's UV spectra placed out of range of UV-vis Perkin spectrophotometer. So it is not possible to calculate by Tauc...

09 November 2016 1,452 4 View

How can I calculate the area of hysteresis in cyclic loading?

I have force and displacement data of 15 loop of cyclic loading in excel file. How can I calculate the Hysteresis area in every cycle (the area between loading and unloading in cyclic loading in...

13 December 2014 1,540 17 View

What is the maximum length of DNA which can be transformed by Agrobacterium tumefaciens?

17 September 2014 1,596 7 View

What is diffrence between coupling buffer and immobilization buffer in SPR experiment?

i am performing SPR experiment and i am referring some journal and websites they stated regarding coupling buffer and immobilization buffer so ihave confusion about these buffer.

06 December 2020 470 3 View

How can the efficiency of the coupling between the incident light and plasmonic coupling can be calculated of a PCF based SPR sensor?

I am working on a PCF based plasmonic sensor for refractive index detection. The work is simulation based using COMSOL Multiphysics software. Theoretically how can I calculate the efficiency of...

29 November 2020 2,575 4 View

Does anyone have experience with the Nicoya OpenSPR COOH sensors?

Hi, I am planning on running some protein-protein interaction/kinetics studies using the Nicoya COOH sensors in OpenSPR. I am looking for the activation and blocking buffer composition that are...

01 November 2020 262 1 View

What is a proper protocol i can follow for an experiment regarding nano fabrication of hollow structures in SPR?

what is a proper protocol i can follow for an experiment regarding nano fabrication of hollow structures in SPR? , for example the methods and the instruments needed, chemical solutions, etc, and...

05 September 2020 4,468 3 View

Hello Everyone, Can anyone suggest me publications or study material on microfluidics for SPR biosensors?

Hi, I am looking for substantial evidence of effect of geomerty of flow channels (microfluidics) on the sensitivity and feasibility of Surface Plasmon Resonance (SPR) Biosensors with respect to...

31 August 2020 6,049 3 View

Which are the main limitations comparing SPR with BLI, especially in terms of buffer and matrix ?

Which are the main limitations of SPR comparing with BLI, especially in terms of buffer and matrix especially for liposome-protein interactions assays ?

13 August 2020 2,194 3 View

How to evaluate affinity of antibody -coated nanoparticle ? SPR/Blitz can help?

I Want to evaluate the binding affinity characterization and kinetic binding of the antibody coated on the nanoparticle. SPR, Blitz based experiments could help?

28 June 2020 8,579 3 View

Immobilizing an antibody with Protein A/G for SPR?

I am planning to perform an antibody-ligand interaction study with SPR/Biacore. The idea is to immobilize my antibody with Protein A/G. It would be great if you could share your comments, what to...

23 June 2020 7,705 3 View

Do buffers need to match for DNA (aptamer) to protein binding in SPR on NTA chip?

Hello, As a newbie to SPR, I've been having problems with optimizing aptamer-protein binding on an NTA chip. My protein has a his-tag on it. Any help would be greatly appreciated. Question 1:...

14 June 2020 4,586 3 View

Why we did not use normal glass (silica glass)in the surface plasmon resonance(SPR) based sensor ??

I have designed a SPR chip by using normal glass slide(Blue star No. 4slide)/Cu(5nm)/Ag(50nm). But it shows very less depth in the reflectance curve at resonance angle. What should be the reason ??

13 February 2020 3,770 4 View