Can I make a primerset (for RT-qPCR) for one single transcript variant? If yes, how?

I am trying to perform a RT-qPCR and I would like to design a primerset for one single transcript variant for RT-qPCR. The problem is that it is very difficult to get primers that only bind on one...

22 April 2020 3,071 4 View

How do I calculate effect size for mixed model regression (run using MIXED in SPSS) when there's only one group? So I can report the effect of time?

I have one group of people who completed measures at 3 time points and I'd like to calculate the effect size of change over time.

15 August 2018 9,774 4 View

Looking for a questionnaire to measure body perception in view of mindfulness and resilience.

05 April 2017 5,427 3 View

How do I quickly find out whether an AAV plasmid is single-stranded or self-complementary?

Hi all, We have purchased an Addgene plasmid (#61593) and cannot find out in articles referencing it (have not emailed the authors yet) whether this is an ss or sc vector. What is the quickest...

18 October 2016 6,262 2 View

How much would glycosylation affect the size of a 122kDa protein?

12 January 2016 2,942 7 View

Can anyone help with AAV transduction in SH-SY5Y cells?

06 August 2015 5,944 10 View

Nuclear membrane lysis buffer compatible with downstream immunoprecipitation (IP)?

I am performing cytoplasmic/nuclear fractionation of HeLa cells with the objective of performing IP using antibody-conjugated magnetic beads on both fractions. I need to make sure I am not...

10 October 2013 621 1 View

Transfection of human iPS cells: is it possible and what should one be aware of?

I would like to perform over-expression experiments of tagged proteins using pcDNA3.1 as a vector. Keyword searches unvariably give me information on the use of vectors to induce pluripotency, but...

03 October 2013 2,023 9 View

Is anyone experienced with the box-counting method of fractal analysis?

We are currently developing a program to analyse the visual complexity of architecture using the box counting method of computational fractal analysis. We are interested to hear of others...

17 January 2013 7,182 37 View

What are the advisable methods to prevent missing out on colistin resistance after the isolates are confirmed as colistin resistant, from sampling?

Generally, we tend to get the phenotypic resistance to colistin in a large percentage. However, when screening for the mcr-1 gene, the percentage recovered, is very less. At times even the...

01 January 1970 295 2 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

APA 7th In-Text citations with two references with three authors with the same surname, but four different author?

I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...

02 March 2021 790 3 View

How Download/Extract citations of 1 given year ALL my publications in Google Scholar?

If you google this you find a lot of smart guys explaining you how to download 1 year of citations of 1 single paper of yours (or anyone else). I mean 1 year of ALL my papers. or ... ALL years or...

01 March 2021 1,121 9 View

No title

Hi, I have several doubts about one paper (Systematic Literature Review) that was rejected. The main topic is Single-Board Computers in engineering education during the period 2010-2020. The...

01 March 2021 9,015 3 View

Which software is better to write systematic review paper?

We have seen some authors use some software to write review paper. As obvious, the study of all published papers concerning our studying field is not possible and these software can help us to...

27 February 2021 6,296 1 View

Is there a fast method matching citations to bibliography (manually typed references)?

I need to be able to match in-text citations to a reference list and back again in very large documents (100+ pages) WITHOUT the use of referencing software like Endnote. my method is to type the...

27 February 2021 9,848 1 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

Centrality measures computation in Biblioshiny/ Vosviewer?

26 February 2021 7,279 2 View

What is the most important parameter of a research work, the impact factor or the h index?

The impact factor is a measure of the importance of a journal based on the number of citations received in a year. ... Articles published in a journal with a high impact factor do not all receive...

24 February 2021 7,481 2 View